Results 121 - 129 of 129 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33366 | 5' | -61.6 | NC_007605.1 | + | 133048 | 0.67 | 0.618732 |
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Target: 5'- aACGCCGccaagacuuccaGGCCCUGAcugcccaGCAcggGGgGGCa -3' miRNA: 3'- gUGCGGCa-----------CCGGGACU-------CGUa--CCgCCG- -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 115509 | 0.67 | 0.611865 |
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Target: 5'- aGgGCCGgGGCCgaGAcCAaGGCGGCu -3' miRNA: 3'- gUgCGGCaCCGGgaCUcGUaCCGCCG- -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 47266 | 0.68 | 0.553504 |
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Target: 5'- gAC-CCGcgaGGCCuuCUGGGCGuUGGCGGCc -3' miRNA: 3'- gUGcGGCa--CCGG--GACUCGU-ACCGCCG- -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 54025 | 0.68 | 0.534388 |
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Target: 5'- -cCGCCcUGGCCC-GGGCA--GCGGCc -3' miRNA: 3'- guGCGGcACCGGGaCUCGUacCGCCG- -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 3009 | 0.69 | 0.524917 |
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Target: 5'- uGgGCUGUaGGCCCucuccucccuUGAGCGUGGCGu- -3' miRNA: 3'- gUgCGGCA-CCGGG----------ACUCGUACCGCcg -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 12388 | 0.69 | 0.521146 |
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Target: 5'- cUugGCCGggucuaagguggccUGGCCCgggGuGCGUGGCcaagaggGGCa -3' miRNA: 3'- -GugCGGC--------------ACCGGGa--CuCGUACCG-------CCG- -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 15457 | 0.69 | 0.521146 |
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Target: 5'- cUugGCCGggucuaagguggccUGGCCCgggGuGCGUGGCcaagaggGGCa -3' miRNA: 3'- -GugCGGC--------------ACCGGGa--CuCGUACCG-------CCG- -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 18526 | 0.69 | 0.521146 |
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Target: 5'- cUugGCCGggucuaagguggccUGGCCCgggGuGCGUGGCcaagaggGGCa -3' miRNA: 3'- -GugCGGC--------------ACCGGGa--CuCGUACCG-------CCG- -5' |
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| 33366 | 5' | -61.6 | NC_007605.1 | + | 135016 | 1.09 | 0.00094 |
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Target: 5'- gCACGCCGUGGCCCUGAGCAUGGCGGCc -3' miRNA: 3'- -GUGCGGCACCGGGACUCGUACCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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