Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
33390 | 3' | -41.7 | NC_007605.1 | + | 148534 | 0.72 | 0.999605 |
Target: 5'- uGUCCCUGggcgUUGCUGCAGCc---- -3' miRNA: 3'- -UAGGGAU----GAUGACGUCGuaaau -5' |
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33390 | 3' | -41.7 | NC_007605.1 | + | 160729 | 0.73 | 0.999186 |
Target: 5'- uGUCCCUGgaGCUGguGCAc--- -3' miRNA: 3'- -UAGGGAUgaUGACguCGUaaau -5' |
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33390 | 3' | -41.7 | NC_007605.1 | + | 99900 | 0.73 | 0.998431 |
Target: 5'- aGUCCCUGaaccuCUGCAGCAUc-- -3' miRNA: 3'- -UAGGGAUgau--GACGUCGUAaau -5' |
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33390 | 3' | -41.7 | NC_007605.1 | + | 148823 | 1.04 | 0.095766 |
Target: 5'- gAUCCCUACUACUGCAGCAUUUAc -3' miRNA: 3'- -UAGGGAUGAUGACGUCGUAAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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