Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33390 | 3' | -41.7 | NC_007605.1 | + | 62200 | 0.66 | 1 |
Target: 5'- -aCCCUGCc-CUGCAGCc---- -3' miRNA: 3'- uaGGGAUGauGACGUCGuaaau -5' |
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33390 | 3' | -41.7 | NC_007605.1 | + | 114442 | 0.67 | 1 |
Target: 5'- uUCCCgGCUACUguucGCGGCGg--- -3' miRNA: 3'- uAGGGaUGAUGA----CGUCGUaaau -5' |
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33390 | 3' | -41.7 | NC_007605.1 | + | 51367 | 0.66 | 1 |
Target: 5'- cUCCCaACUGCUGgAGCc---- -3' miRNA: 3'- uAGGGaUGAUGACgUCGuaaau -5' |
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33390 | 3' | -41.7 | NC_007605.1 | + | 113932 | 0.66 | 1 |
Target: 5'- uGUCCUUGuCUGCUGCcauGCAc--- -3' miRNA: 3'- -UAGGGAU-GAUGACGu--CGUaaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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