Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 334 | 3' | -54.8 | AC_000010.1 | + | 6933 | 0.66 | 0.562222 |
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Target: 5'- cGC-CGAGCUGGUgGCCaaGCuGCAAGa -3' miRNA: 3'- -UGaGUUCGGUCAgUGGgaCGuCGUUC- -5' |
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| 334 | 3' | -54.8 | AC_000010.1 | + | 2026 | 0.66 | 0.550835 |
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Target: 5'- uACU--GGCCAGUacaGCCUcugggUGUAGCAGGg -3' miRNA: 3'- -UGAguUCGGUCAg--UGGG-----ACGUCGUUC- -5' |
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| 334 | 3' | -54.8 | AC_000010.1 | + | 10207 | 0.66 | 0.539519 |
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Target: 5'- cGCUCuguAGCCGGggCGCCa-GgAGCGAGg -3' miRNA: 3'- -UGAGu--UCGGUCa-GUGGgaCgUCGUUC- -5' |
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| 334 | 3' | -54.8 | AC_000010.1 | + | 5039 | 0.67 | 0.517135 |
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Target: 5'- aGCUCAAGCCGGUC-CCagaGCucGGUAAu -3' miRNA: 3'- -UGAGUUCGGUCAGuGGga-CG--UCGUUc -5' |
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| 334 | 3' | -54.8 | AC_000010.1 | + | 1348 | 0.69 | 0.402124 |
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Target: 5'- aGCUUAAGCCuGggaaaCGCCCUGCuGUGAa -3' miRNA: 3'- -UGAGUUCGGuCa----GUGGGACGuCGUUc -5' |
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| 334 | 3' | -54.8 | AC_000010.1 | + | 21890 | 0.69 | 0.364494 |
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Target: 5'- --cCAAGuCCAGccCACCCUGC-GCAGGa -3' miRNA: 3'- ugaGUUC-GGUCa-GUGGGACGuCGUUC- -5' |
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| 334 | 3' | -54.8 | AC_000010.1 | + | 10024 | 0.74 | 0.186042 |
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Target: 5'- gACUucCAAGCCAcccUCACCCaggUGCAGCAAGc -3' miRNA: 3'- -UGA--GUUCGGUc--AGUGGG---ACGUCGUUC- -5' |
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| 334 | 3' | -54.8 | AC_000010.1 | + | 16654 | 1.05 | 0.000959 |
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Target: 5'- cCUCAAGCCAGUCACCCUGCAGCAAGu -3' miRNA: 3'- uGAGUUCGGUCAGUGGGACGUCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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