Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 334 | 5' | -56.6 | AC_000010.1 | + | 18123 | 0.66 | 0.470256 |
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Target: 5'- cUGCCUCCuCGUAGgaGGGCggGUCGucugAGCGu -3' miRNA: 3'- -ACGGAGGuGCGUC--UCCG--UAGU----UCGCu -5' |
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| 334 | 5' | -56.6 | AC_000010.1 | + | 13861 | 0.72 | 0.209953 |
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Target: 5'- cGCC-CaACGCGGGGGCG-CAGGUGAg -3' miRNA: 3'- aCGGaGgUGCGUCUCCGUaGUUCGCU- -5' |
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| 334 | 5' | -56.6 | AC_000010.1 | + | 23674 | 0.72 | 0.198683 |
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Target: 5'- gGCCagC-CGCGGAGGaaaucaGUCAAGCGAc -3' miRNA: 3'- aCGGagGuGCGUCUCCg-----UAGUUCGCU- -5' |
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| 334 | 5' | -56.6 | AC_000010.1 | + | 8189 | 0.73 | 0.182768 |
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Target: 5'- uUGCCUCC-CGCGGGGGCuuguuAGCa- -3' miRNA: 3'- -ACGGAGGuGCGUCUCCGuagu-UCGcu -5' |
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| 334 | 5' | -56.6 | AC_000010.1 | + | 16741 | 0.8 | 0.051843 |
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Target: 5'- aUGCCUCUGCGUGGAGGCA-CGGGCa- -3' miRNA: 3'- -ACGGAGGUGCGUCUCCGUaGUUCGcu -5' |
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| 334 | 5' | -56.6 | AC_000010.1 | + | 16688 | 1.09 | 0.00034 |
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Target: 5'- gUGCCUCCACGCAGAGGCAUCAAGCGAg -3' miRNA: 3'- -ACGGAGGUGCGUCUCCGUAGUUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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