Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 33401 | 3' | -50.7 | NC_007605.1 | + | 125317 | 0.73 | 0.861625 |
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Target: 5'- -----gGUGGGCUGCGGUugcaGGCCAggGCg -3' miRNA: 3'- agguaaUACCCGAUGCCG----UUGGUa-CG- -5' |
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| 33401 | 3' | -50.7 | NC_007605.1 | + | 72587 | 0.75 | 0.733214 |
|
Target: 5'- gUCCAccUUGUaGGuaUGCGGCAGCCGgaUGCa -3' miRNA: 3'- -AGGU--AAUA-CCcgAUGCCGUUGGU--ACG- -5' |
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| 33401 | 3' | -50.7 | NC_007605.1 | + | 102467 | 0.8 | 0.493943 |
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Target: 5'- cUCUuggGUUAUGGGCUucuuaACGGCGggcACCAUGCc -3' miRNA: 3'- -AGG---UAAUACCCGA-----UGCCGU---UGGUACG- -5' |
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| 33401 | 3' | -50.7 | NC_007605.1 | + | 49385 | 0.83 | 0.366403 |
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Target: 5'- cUCCAgccuccccuggaggGUGGGCUugGGCAGCCG-GCg -3' miRNA: 3'- -AGGUaa------------UACCCGAugCCGUUGGUaCG- -5' |
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| 33401 | 3' | -50.7 | NC_007605.1 | + | 155735 | 1.12 | 0.006023 |
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Target: 5'- uUCCAUUAUGGGCUACGGCAACCAUGCc -3' miRNA: 3'- -AGGUAAUACCCGAUGCCGUUGGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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