Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33414 | 3' | -58.2 | NC_007605.1 | + | 115915 | 0.68 | 0.740487 |
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Target: 5'- --aCGAgGAGGgGGGUCC-CGaGGCCAc -3' miRNA: 3'- guaGCUgUUCC-CCCAGGaGUgCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 153232 | 0.68 | 0.740487 |
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Target: 5'- -cUCGGCGuAGGccGGGUCCU--CGGCCAu -3' miRNA: 3'- guAGCUGU-UCC--CCCAGGAguGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 115489 | 0.68 | 0.740487 |
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Target: 5'- aCAUCaggGACAAGGuGG-CCUCGgCGGCCu -3' miRNA: 3'- -GUAG---CUGUUCCcCCaGGAGU-GCCGGu -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 112737 | 0.69 | 0.707669 |
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Target: 5'- -cUgGACGAGGGGGcgcggcuggaugcCCUCAUGcGCCAa -3' miRNA: 3'- guAgCUGUUCCCCCa------------GGAGUGC-CGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 168781 | 0.7 | 0.612252 |
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Target: 5'- ---aGGagaGGGGGGGUCCUCGagggGGCCGu -3' miRNA: 3'- guagCUg--UUCCCCCAGGAGUg---CCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 40208 | 0.73 | 0.485923 |
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Target: 5'- --cCGACuggcAGGGGGUCCcCAUGGCaCAg -3' miRNA: 3'- guaGCUGu---UCCCCCAGGaGUGCCG-GU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 155591 | 0.73 | 0.440565 |
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Target: 5'- --cCGGC-AGGGGGUCCU-GCGGUCGa -3' miRNA: 3'- guaGCUGuUCCCCCAGGAgUGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 85548 | 0.75 | 0.349959 |
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Target: 5'- ---gGGCAAGGGGGUUCcccaACGGCCAu -3' miRNA: 3'- guagCUGUUCCCCCAGGag--UGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 9502 | 1.08 | 0.002393 |
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Target: 5'- cCAUCGACAAGGGGGUCCUCACGGCCAa -3' miRNA: 3'- -GUAGCUGUUCCCCCAGGAGUGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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