Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33414 | 3' | -58.2 | NC_007605.1 | + | 38335 | 0.66 | 0.827249 |
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Target: 5'- --cCGugGAGGGGGaCCUgACcugcaugcucuccaGGCCAu -3' miRNA: 3'- guaGCugUUCCCCCaGGAgUG--------------CCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 76536 | 0.67 | 0.795646 |
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Target: 5'- --aCGGCGGGGGaGGuuUCCUCAggcuUGGCUAa -3' miRNA: 3'- guaGCUGUUCCC-CC--AGGAGU----GCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 16406 | 0.67 | 0.777734 |
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Target: 5'- aUAUCGAguGucuGGGGGaggagggcuUCCcCACGGCCAc -3' miRNA: 3'- -GUAGCUguU---CCCCC---------AGGaGUGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 109227 | 0.68 | 0.749955 |
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Target: 5'- uCAUCGAaGAGGGcagcggggaGGUgCUCGCGGUCu -3' miRNA: 3'- -GUAGCUgUUCCC---------CCAgGAGUGCCGGu -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 96230 | 0.68 | 0.740487 |
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Target: 5'- --gUGACGcGGGGGUCCU--UGGCCc -3' miRNA: 3'- guaGCUGUuCCCCCAGGAguGCCGGu -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 115489 | 0.68 | 0.740487 |
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Target: 5'- aCAUCaggGACAAGGuGG-CCUCGgCGGCCu -3' miRNA: 3'- -GUAG---CUGUUCCcCCaGGAGU-GCCGGu -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 112737 | 0.69 | 0.707669 |
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Target: 5'- -cUgGACGAGGGGGcgcggcuggaugcCCUCAUGcGCCAa -3' miRNA: 3'- guAgCUGUUCCCCCa------------GGAGUGC-CGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 9502 | 1.08 | 0.002393 |
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Target: 5'- cCAUCGACAAGGGGGUCCUCACGGCCAa -3' miRNA: 3'- -GUAGCUGUUCCCCCAGGAGUGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 157849 | 0.66 | 0.84887 |
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Target: 5'- aCAUCugGACAAaguuugggggcgcccGGGGGUCgggCugGGCCGc -3' miRNA: 3'- -GUAG--CUGUU---------------CCCCCAGga-GugCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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