Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33414 | 3' | -58.2 | NC_007605.1 | + | 115915 | 0.68 | 0.740487 |
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Target: 5'- --aCGAgGAGGgGGGUCC-CGaGGCCAc -3' miRNA: 3'- guaGCUgUUCC-CCCAGGaGUgCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 153232 | 0.68 | 0.740487 |
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Target: 5'- -cUCGGCGuAGGccGGGUCCU--CGGCCAu -3' miRNA: 3'- guAGCUGU-UCC--CCCAGGAguGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 123954 | 0.68 | 0.758393 |
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Target: 5'- gAUCGGCAcccGGGGcugaUCCUCgaccccuGCGGCCAc -3' miRNA: 3'- gUAGCUGUu--CCCCc---AGGAG-------UGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 157431 | 0.67 | 0.795646 |
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Target: 5'- --aCGGCGGcGGGGGUgCUCccGCgGGCCGc -3' miRNA: 3'- guaGCUGUU-CCCCCAgGAG--UG-CCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 67887 | 0.66 | 0.853494 |
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Target: 5'- --gCGgccACAAGGGGGUUC-C-CGGCCu -3' miRNA: 3'- guaGC---UGUUCCCCCAGGaGuGCCGGu -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 63109 | 0.66 | 0.8588 |
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Target: 5'- ---aGugGAGGGGGgagCUUCagcuggucugggugGCGGCCGc -3' miRNA: 3'- guagCugUUCCCCCa--GGAG--------------UGCCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 61976 | 0.66 | 0.861045 |
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Target: 5'- gCcgCGACGGGGGGcguuuGUCCccCGCuGGCCu -3' miRNA: 3'- -GuaGCUGUUCCCC-----CAGGa-GUG-CCGGu -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 73466 | 0.66 | 0.861045 |
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Target: 5'- uCAUCGGgGucGGGGUCC-CACguguauggGGCCGg -3' miRNA: 3'- -GUAGCUgUucCCCCAGGaGUG--------CCGGU- -5' |
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| 33414 | 3' | -58.2 | NC_007605.1 | + | 85548 | 0.75 | 0.349959 |
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Target: 5'- ---gGGCAAGGGGGUUCcccaACGGCCAu -3' miRNA: 3'- guagCUGUUCCCCCAGGag--UGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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