Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33420 | 5' | -54.1 | NC_007605.1 | + | 104679 | 0.66 | 0.958525 |
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Target: 5'- gCAGGGUGACggcggcCGC-CACCuugucacggagcCAGGAAAu -3' miRNA: 3'- aGUCCCACUGa-----GCGaGUGG------------GUUCUUU- -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 116427 | 0.67 | 0.931768 |
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Target: 5'- aUCAGGGUGcACUCGCUggAUCCAu---- -3' miRNA: 3'- -AGUCCCAC-UGAGCGAg-UGGGUucuuu -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 168582 | 0.68 | 0.902706 |
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Target: 5'- cCGGGG-GGCgCGCguaGCCCGAGAu- -3' miRNA: 3'- aGUCCCaCUGaGCGag-UGGGUUCUuu -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 67454 | 0.68 | 0.889372 |
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Target: 5'- gUCAGGGUGGCUa-CUUGCUCAGGu-- -3' miRNA: 3'- -AGUCCCACUGAgcGAGUGGGUUCuuu -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 34848 | 0.68 | 0.889372 |
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Target: 5'- -gAGGGUGGCgUC-CUCcCCCGGGAAu -3' miRNA: 3'- agUCCCACUG-AGcGAGuGGGUUCUUu -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 35137 | 0.68 | 0.875098 |
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Target: 5'- cCGGGGcGAC-CGCUCcggcCCCAGGAc- -3' miRNA: 3'- aGUCCCaCUGaGCGAGu---GGGUUCUuu -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 49575 | 0.71 | 0.772444 |
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Target: 5'- cCGGGaGUGGCacCGCUCGCCCAuGGGc -3' miRNA: 3'- aGUCC-CACUGa-GCGAGUGGGUuCUUu -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 104199 | 0.71 | 0.743252 |
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Target: 5'- -uGGGGUGcCUgGCcaggUCACCCAAGGAu -3' miRNA: 3'- agUCCCACuGAgCG----AGUGGGUUCUUu -5' |
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| 33420 | 5' | -54.1 | NC_007605.1 | + | 6678 | 1.07 | 0.005701 |
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Target: 5'- gUCAGGGUGACUCGCUCACCCAAGAAAg -3' miRNA: 3'- -AGUCCCACUGAGCGAGUGGGUUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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