Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 335 | 3' | -56.1 | AC_000010.1 | + | 29632 | 0.67 | 0.504993 |
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Target: 5'- gCGGGGAGcUGga--GUGGUGGCUG-GCa -3' miRNA: 3'- -GCCUCUC-ACauagCGCUACCGGCgCG- -5' |
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| 335 | 3' | -56.1 | AC_000010.1 | + | 23519 | 0.67 | 0.494436 |
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Target: 5'- gCGGAG-GUGac-UGCGAUgGGCUGCGg -3' miRNA: 3'- -GCCUCuCACauaGCGCUA-CCGGCGCg -5' |
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| 335 | 3' | -56.1 | AC_000010.1 | + | 67 | 0.67 | 0.473632 |
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Target: 5'- gGGAGGGUGaAagGUGAuUGGCUGUGg -3' miRNA: 3'- gCCUCUCACaUagCGCU-ACCGGCGCg -5' |
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| 335 | 3' | -56.1 | AC_000010.1 | + | 35458 | 0.67 | 0.473632 |
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Target: 5'- gGGAGGGUGaAagGUGAuUGGCUGUGg -3' miRNA: 3'- gCCUCUCACaUagCGCU-ACCGGCGCg -5' |
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| 335 | 3' | -56.1 | AC_000010.1 | + | 23587 | 0.68 | 0.443279 |
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Target: 5'- uCGG-GGGUGUGcUCcCGGUGGCgGuCGCu -3' miRNA: 3'- -GCCuCUCACAU-AGcGCUACCGgC-GCG- -5' |
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| 335 | 3' | -56.1 | AC_000010.1 | + | 9154 | 0.72 | 0.265719 |
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Target: 5'- aCGGAuGAGU---UCgGCGAUGGUgGCGCg -3' miRNA: 3'- -GCCU-CUCAcauAG-CGCUACCGgCGCG- -5' |
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| 335 | 3' | -56.1 | AC_000010.1 | + | 17214 | 1.12 | 0.000297 |
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Target: 5'- gCGGAGAGUGUAUCGCGAUGGCCGCGCg -3' miRNA: 3'- -GCCUCUCACAUAGCGCUACCGGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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