Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
335 | 5' | -54.1 | AC_000010.1 | + | 21676 | 0.66 | 0.614854 |
Target: 5'- aGGgGUUGCUGCGcCGUaGCGCccuUGCUACu -3' miRNA: 3'- aCUgCAACGGUGC-GCA-UGCG---AUGGUG- -5' |
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335 | 5' | -54.1 | AC_000010.1 | + | 25297 | 0.66 | 0.614854 |
Target: 5'- ------cGCCACcuGCuGUGCGCUACCAUc -3' miRNA: 3'- acugcaaCGGUG--CG-CAUGCGAUGGUG- -5' |
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335 | 5' | -54.1 | AC_000010.1 | + | 17391 | 0.67 | 0.569176 |
Target: 5'- gGAUGUUGggGCGCGggAUGCgacGCCACa -3' miRNA: 3'- aCUGCAACggUGCGCa-UGCGa--UGGUG- -5' |
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335 | 5' | -54.1 | AC_000010.1 | + | 17248 | 1.09 | 0.000701 |
Target: 5'- aUGACGUUGCCACGCGUACGCUACCACc -3' miRNA: 3'- -ACUGCAACGGUGCGCAUGCGAUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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