Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33513 | 3' | -58.8 | NC_007605.1 | + | 111120 | 0.66 | 0.787476 |
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Target: 5'- gGCCUGggucuCUCGA-GGgGGUucUGGGGAGAc -3' miRNA: 3'- -UGGAC-----GAGCUaCCgCCG--ACCUCUCUu -5' |
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| 33513 | 3' | -58.8 | NC_007605.1 | + | 61343 | 0.66 | 0.778323 |
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Target: 5'- cACCUGCucauacUCGGccGCGGgaGGGGGGAc -3' miRNA: 3'- -UGGACG------AGCUacCGCCgaCCUCUCUu -5' |
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| 33513 | 3' | -58.8 | NC_007605.1 | + | 135576 | 0.66 | 0.778323 |
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Target: 5'- cGCCagUGCUgGGUuacuGCGGgUGGAGGGAAa -3' miRNA: 3'- -UGG--ACGAgCUAc---CGCCgACCUCUCUU- -5' |
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| 33513 | 3' | -58.8 | NC_007605.1 | + | 60037 | 0.67 | 0.740509 |
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Target: 5'- cGCUcgGCgUCGAUGGCGGgUGGcuGGAAc -3' miRNA: 3'- -UGGa-CG-AGCUACCGCCgACCucUCUU- -5' |
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| 33513 | 3' | -58.8 | NC_007605.1 | + | 49877 | 0.68 | 0.671096 |
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Target: 5'- uCCUGUUCcggGGCGGCggUGGAuGAGAGg -3' miRNA: 3'- uGGACGAGcuaCCGCCG--ACCU-CUCUU- -5' |
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| 33513 | 3' | -58.8 | NC_007605.1 | + | 117005 | 0.74 | 0.329957 |
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Target: 5'- cCCUGCUcguggaCGAUGGUGGUgcggcGGAGAGAAu -3' miRNA: 3'- uGGACGA------GCUACCGCCGa----CCUCUCUU- -5' |
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| 33513 | 3' | -58.8 | NC_007605.1 | + | 57963 | 0.75 | 0.301313 |
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Target: 5'- cGCC-GCUCGAcgcccuUGGUGGCgUGGAGGGAGc -3' miRNA: 3'- -UGGaCGAGCU------ACCGCCG-ACCUCUCUU- -5' |
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| 33513 | 3' | -58.8 | NC_007605.1 | + | 61742 | 1.05 | 0.002489 |
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Target: 5'- cACCUGCUCGAUGGCGGCUGGAGAGAAg -3' miRNA: 3'- -UGGACGAGCUACCGCCGACCUCUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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