Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33556 | 3' | -53 | NC_007605.1 | + | 147652 | 0.66 | 0.977599 |
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Target: 5'- cCGGUGGUUUCCCUuuuCCGccCGUUUaUCc -3' miRNA: 3'- -GCCACCAAGGGGGu--GGUa-GUAAGaAG- -5' |
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| 33556 | 3' | -53 | NC_007605.1 | + | 60770 | 0.67 | 0.955776 |
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Target: 5'- ---aGGggCCCCCGCCcUCuucgUCUUCu -3' miRNA: 3'- gccaCCaaGGGGGUGGuAGua--AGAAG- -5' |
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| 33556 | 3' | -53 | NC_007605.1 | + | 134334 | 0.67 | 0.955776 |
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Target: 5'- aCGGUGGcggcagaCCCCCAggccuCCAUCAgcucUCUUUu -3' miRNA: 3'- -GCCACCaa-----GGGGGU-----GGUAGUa---AGAAG- -5' |
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| 33556 | 3' | -53 | NC_007605.1 | + | 82073 | 0.69 | 0.922704 |
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Target: 5'- cCGGUGGaUCCUCCACCGcCGguuaaCUUUa -3' miRNA: 3'- -GCCACCaAGGGGGUGGUaGUaa---GAAG- -5' |
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| 33556 | 3' | -53 | NC_007605.1 | + | 111763 | 0.71 | 0.855042 |
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Target: 5'- uGGUGGacgUCCUCACCAUCAUcCUg- -3' miRNA: 3'- gCCACCaa-GGGGGUGGUAGUAaGAag -5' |
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| 33556 | 3' | -53 | NC_007605.1 | + | 105024 | 1.09 | 0.005959 |
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Target: 5'- cCGGUGGUUCCCCCACCAUCAUUCUUCc -3' miRNA: 3'- -GCCACCAAGGGGGUGGUAGUAAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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