Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33593 | 3' | -48 | NC_007605.1 | + | 132063 | 0.66 | 0.999665 |
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Target: 5'- aGGGGCagUAGgcuuUUGGUGUgcggGUGCUGg -3' miRNA: 3'- aCCUUGg-AUCac--AAUCACAa---CACGAC- -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 32642 | 0.66 | 0.999577 |
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Target: 5'- cGGugacACCUGGUGacUAGUGgUGcGCUGg -3' miRNA: 3'- aCCu---UGGAUCACa-AUCACaACaCGAC- -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 137566 | 0.66 | 0.99934 |
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Target: 5'- aUGGGGCCU-GUGacUGGUGcUUGUGgUGu -3' miRNA: 3'- -ACCUUGGAuCACa-AUCAC-AACACgAC- -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 144685 | 0.68 | 0.996758 |
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Target: 5'- gGGGACCcugagacggguggguGUGUUAGUG-UGUGcCUGg -3' miRNA: 3'- aCCUUGGau-------------CACAAUCACaACAC-GAC- -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 139207 | 0.7 | 0.989598 |
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Target: 5'- gGGGGuCUUAGUGgaAGUGacGUGCUGu -3' miRNA: 3'- aCCUU-GGAUCACaaUCACaaCACGAC- -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 167194 | 0.7 | 0.986484 |
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Target: 5'- aGGGgccucaucuGCCUGGUaGUU-GUGUUGUGCa- -3' miRNA: 3'- aCCU---------UGGAUCA-CAAuCACAACACGac -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 66406 | 0.71 | 0.980531 |
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Target: 5'- aGGGACCaGGUGggUGGUG-UGUGCc- -3' miRNA: 3'- aCCUUGGaUCACa-AUCACaACACGac -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 138821 | 0.71 | 0.978167 |
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Target: 5'- gUGGGAaCUAGUGggAGUGcUGUGCc- -3' miRNA: 3'- -ACCUUgGAUCACaaUCACaACACGac -5' |
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| 33593 | 3' | -48 | NC_007605.1 | + | 138943 | 1.09 | 0.016557 |
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Target: 5'- gUGGAACCUAGUGUUAGUGUUGUGCUGu -3' miRNA: 3'- -ACCUUGGAUCACAAUCACAACACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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