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miRNA: 3'- caCCGA--UCUGCAUa-CCGGa-----UGGGUuc -5'
miRNA: 3'- caCCGAUCUGcauACCGGaUGGGUUC- -5'
miRNA: 3'- caCCGAUCUGCAuaCCGgAUGGGUUC- -5'
miRNA: 3'- -CACCGAUCUGCAUACCGGAUGGGUUC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 33599 | 3' | -53.5 | NC_007605.1 | + | 68225 | 0.72 | 0.753096 |
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Target: 5'- -aGGCUcaGGACGUGggGGCCguugagGCCCAc- -3' miRNA: 3'- caCCGA--UCUGCAUa-CCGGa-----UGGGUuc -5' |
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| 33599 | 3' | -53.5 | NC_007605.1 | + | 33918 | 0.73 | 0.682407 |
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Target: 5'- -cGGCUGGGC---UGGCCgGCCCGGGu -3' miRNA: 3'- caCCGAUCUGcauACCGGaUGGGUUC- -5' |
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| 33599 | 3' | -53.5 | NC_007605.1 | + | 54360 | 0.74 | 0.651275 |
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Target: 5'- -aGGCUAGGCGUccGGC-UACUCGGGg -3' miRNA: 3'- caCCGAUCUGCAuaCCGgAUGGGUUC- -5' |
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| 33599 | 3' | -53.5 | NC_007605.1 | + | 140230 | 1.09 | 0.00495 |
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Target: 5'- uGUGGCUAGACGUAUGGCCUACCCAAGa -3' miRNA: 3'- -CACCGAUCUGCAUACCGGAUGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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