Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33599 | 3' | -53.5 | NC_007605.1 | + | 7925 | 0.66 | 0.962153 |
Target: 5'- -gGGCUAGAUcccucuGCCUACCUggGg -3' miRNA: 3'- caCCGAUCUGcauac-CGGAUGGGuuC- -5' |
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33599 | 3' | -53.5 | NC_007605.1 | + | 49144 | 0.66 | 0.965223 |
Target: 5'- -gGGCUgccgucucuaucAGACGcguUAUGGCacgccggCUGCCCAAGc -3' miRNA: 3'- caCCGA------------UCUGC---AUACCG-------GAUGGGUUC- -5' |
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33599 | 3' | -53.5 | NC_007605.1 | + | 160789 | 0.66 | 0.965553 |
Target: 5'- gGUGGCcAGACGgcgcuUGcGUCUGCCCc-- -3' miRNA: 3'- -CACCGaUCUGCau---AC-CGGAUGGGuuc -5' |
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33599 | 3' | -53.5 | NC_007605.1 | + | 74595 | 0.66 | 0.968733 |
Target: 5'- -gGGCUcuGGCucuuUGGCCgGCCCAGGa -3' miRNA: 3'- caCCGAu-CUGcau-ACCGGaUGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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