miRNA: 3'- caCCGaUCUGCaua---CCGGAUG-GGUUC- -5'
miRNA: 3'- -CACCGAUCUgCAUaCCggaUGGGUUC- -5'
miRNA: 3'- cACCGAUCU-GCAuaCCGg---AU-GGGUUC- -5'
miRNA: 3'- -CACCGAUCUGCAUACCGGAUGGGUUC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33599 | 3' | -53.5 | NC_007605.1 | + | 122952 | 0.67 | 0.946238 |
|
Target: 5'- -cGGCcAGGCGcaucagGGCCUGCuCUAGGg -3' miRNA: 3'- caCCGaUCUGCaua---CCGGAUG-GGUUC- -5' |
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| 33599 | 3' | -53.5 | NC_007605.1 | + | 92086 | 0.67 | 0.946238 |
|
Target: 5'- cGUGGCUGGA-GUA-GGag-GCCCGGGg -3' miRNA: 3'- -CACCGAUCUgCAUaCCggaUGGGUUC- -5' |
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| 33599 | 3' | -53.5 | NC_007605.1 | + | 115753 | 0.68 | 0.926451 |
|
Target: 5'- aUGGCUAGGcCGUccGGCagcaUGcCCCGAGc -3' miRNA: 3'- cACCGAUCU-GCAuaCCGg---AU-GGGUUC- -5' |
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| 33599 | 3' | -53.5 | NC_007605.1 | + | 140230 | 1.09 | 0.00495 |
|
Target: 5'- uGUGGCUAGACGUAUGGCCUACCCAAGa -3' miRNA: 3'- -CACCGAUCUGCAUACCGGAUGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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