Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33599 | 5' | -52.5 | NC_007605.1 | + | 84306 | 0.66 | 0.977181 |
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Target: 5'- cGUCUUGGGcgUGGGCCAaGGcagUUUCCu -3' miRNA: 3'- -CAGAGCCC--AUCCGGUaCUaa-GAAGGu -5' |
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| 33599 | 5' | -52.5 | NC_007605.1 | + | 45088 | 0.67 | 0.971869 |
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Target: 5'- cGUC-CGGGUGGGCCccGAggCUcUCgCAg -3' miRNA: 3'- -CAGaGCCCAUCCGGuaCUaaGA-AG-GU- -5' |
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| 33599 | 5' | -52.5 | NC_007605.1 | + | 153231 | 0.67 | 0.965721 |
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Target: 5'- -gCUCGGcGUAGGCCG-GGUccUCggCCAu -3' miRNA: 3'- caGAGCC-CAUCCGGUaCUA--AGaaGGU- -5' |
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| 33599 | 5' | -52.5 | NC_007605.1 | + | 45947 | 0.73 | 0.732369 |
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Target: 5'- aGUggUGGGUAGGCUAUGccUUCUUCCGc -3' miRNA: 3'- -CAgaGCCCAUCCGGUACu-AAGAAGGU- -5' |
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| 33599 | 5' | -52.5 | NC_007605.1 | + | 31394 | 0.78 | 0.466716 |
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Target: 5'- cGUCUUGGGUAGGCCAUacgUCUagCCAc -3' miRNA: 3'- -CAGAGCCCAUCCGGUAcuaAGAa-GGU- -5' |
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| 33599 | 5' | -52.5 | NC_007605.1 | + | 140265 | 1.09 | 0.006583 |
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Target: 5'- gGUCUCGGGUAGGCCAUGAUUCUUCCAg -3' miRNA: 3'- -CAGAGCCCAUCCGGUACUAAGAAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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