Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33602 | 5' | -51.2 | NC_007605.1 | + | 170797 | 0.69 | 0.946761 |
Target: 5'- --gGCAUgGuUAGUCCuGUGUCCAGa- -3' miRNA: 3'- uuaCGUAgU-AUCAGGuCACAGGUCcc -5' |
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33602 | 5' | -51.2 | NC_007605.1 | + | 51382 | 0.7 | 0.921049 |
Target: 5'- -uUGCAUCAUGGUgacCCuGcUGcCCAGGGc -3' miRNA: 3'- uuACGUAGUAUCA---GGuC-ACaGGUCCC- -5' |
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33602 | 5' | -51.2 | NC_007605.1 | + | 82628 | 0.72 | 0.850815 |
Target: 5'- gGAUGCAUCGUAGUC-AGUcugcuucgcGUCCuuuGGGa -3' miRNA: 3'- -UUACGUAGUAUCAGgUCA---------CAGGu--CCC- -5' |
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33602 | 5' | -51.2 | NC_007605.1 | + | 98728 | 0.84 | 0.279131 |
Target: 5'- cAUGCGcCGccugGGUCCAGUGUCCAGGGc -3' miRNA: 3'- uUACGUaGUa---UCAGGUCACAGGUCCC- -5' |
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33602 | 5' | -51.2 | NC_007605.1 | + | 44537 | 0.66 | 0.987634 |
Target: 5'- --aGCGUUAcGGcCCAGUauGUCCAGGcGg -3' miRNA: 3'- uuaCGUAGUaUCaGGUCA--CAGGUCC-C- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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