miRNA: 3'- uacCGuaCuuCCGuGuCGGACA---AUGG- -5'
miRNA: 3'- -UACCGUaCUUCCg-UGUCGga-CAAUGG- -5'
miRNA: 3'- uaCCGUaCUUCCGugUCGgaCaa----UGG- -5'
miRNA: 3'- uaCCGuaCuuCCGUGuCGGaCaa---UGG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33605 | 5' | -52.5 | NC_007605.1 | + | 159254 | 0.66 | 0.980725 |
|
Target: 5'- --uGCcgGggGGCcCuGCCUGUcucUGCCc -3' miRNA: 3'- uacCGuaCuuCCGuGuCGGACA---AUGG- -5' |
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| 33605 | 5' | -52.5 | NC_007605.1 | + | 159573 | 0.72 | 0.782606 |
|
Target: 5'- gGUGGCAgGAGGGgaGCAGCaggGUUGCUg -3' miRNA: 3'- -UACCGUaCUUCCg-UGUCGga-CAAUGG- -5' |
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| 33605 | 5' | -52.5 | NC_007605.1 | + | 162895 | 0.66 | 0.980725 |
|
Target: 5'- -gGGCAaGAAGGUugGGCgaGaaggagGCCg -3' miRNA: 3'- uaCCGUaCUUCCGugUCGgaCaa----UGG- -5' |
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| 33605 | 5' | -52.5 | NC_007605.1 | + | 169644 | 0.67 | 0.96726 |
|
Target: 5'- -gGGUcgGggGGCGCcGCCgGgcgcaGCCa -3' miRNA: 3'- uaCCGuaCuuCCGUGuCGGaCaa---UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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