Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33606 | 3' | -50.9 | NC_007605.1 | + | 155715 | 0.69 | 0.961185 |
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Target: 5'- cGCUgGCccCaUCGGccuuGCGCCGGCCCAGu -3' miRNA: 3'- -CGA-UGa-GaAGCCuu--UGCGGUUGGGUC- -5' |
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| 33606 | 3' | -50.9 | NC_007605.1 | + | 54373 | 0.7 | 0.944906 |
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Target: 5'- gGCUACUCgggggUUGaGggUGUCAACCCGGc -3' miRNA: 3'- -CGAUGAGa----AGCcUuuGCGGUUGGGUC- -5' |
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| 33606 | 3' | -50.9 | NC_007605.1 | + | 95859 | 0.7 | 0.944906 |
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Target: 5'- gGCUGC-CUUCaGAuAUGCCAcccCCCAGa -3' miRNA: 3'- -CGAUGaGAAGcCUuUGCGGUu--GGGUC- -5' |
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| 33606 | 3' | -50.9 | NC_007605.1 | + | 19894 | 0.71 | 0.924697 |
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Target: 5'- cGCUGCUCUUCcugGGuGAgGCCGggaGCCUGGg -3' miRNA: 3'- -CGAUGAGAAG---CCuUUgCGGU---UGGGUC- -5' |
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| 33606 | 3' | -50.9 | NC_007605.1 | + | 122779 | 0.72 | 0.893816 |
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Target: 5'- aCUGCccggaUUCGGGGcCGCCAGCCCGa -3' miRNA: 3'- cGAUGag---AAGCCUUuGCGGUUGGGUc -5' |
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| 33606 | 3' | -50.9 | NC_007605.1 | + | 148666 | 1.12 | 0.006465 |
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Target: 5'- uGCUACUCUUCGGAAACGCCAACCCAGg -3' miRNA: 3'- -CGAUGAGAAGCCUUUGCGGUUGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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