Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33612 | 5' | -52.5 | NC_007605.1 | + | 80068 | 0.66 | 0.987945 |
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Target: 5'- aGCgggUCGaucaugaugCCgGUGGGU-CAGCUCCa -3' miRNA: 3'- -CGacaAGCa--------GGgCACUCAuGUCGAGG- -5' |
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| 33612 | 5' | -52.5 | NC_007605.1 | + | 97277 | 0.66 | 0.986381 |
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Target: 5'- gGCggGUUC-UCUCGUGGaacUGCAGCUUCa -3' miRNA: 3'- -CGa-CAAGcAGGGCACUc--AUGUCGAGG- -5' |
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| 33612 | 5' | -52.5 | NC_007605.1 | + | 124219 | 0.67 | 0.973532 |
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Target: 5'- -gUGUUCcaCCCGUGA--AUGGCUCCc -3' miRNA: 3'- cgACAAGcaGGGCACUcaUGUCGAGG- -5' |
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| 33612 | 5' | -52.5 | NC_007605.1 | + | 99321 | 0.68 | 0.953584 |
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Target: 5'- cCUGgaucUCGUCgCC-UGGGU-CAGCUCCu -3' miRNA: 3'- cGACa---AGCAG-GGcACUCAuGUCGAGG- -5' |
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| 33612 | 5' | -52.5 | NC_007605.1 | + | 78554 | 0.7 | 0.919831 |
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Target: 5'- cGCUGUUCaUCCuUGgggGAGUaguagcuuaGCAGCUCUa -3' miRNA: 3'- -CGACAAGcAGG-GCa--CUCA---------UGUCGAGG- -5' |
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| 33612 | 5' | -52.5 | NC_007605.1 | + | 83226 | 0.72 | 0.835259 |
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Target: 5'- -aUGaUUGgagCCCGUGGGccgGCGGCUCCu -3' miRNA: 3'- cgACaAGCa--GGGCACUCa--UGUCGAGG- -5' |
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| 33612 | 5' | -52.5 | NC_007605.1 | + | 156759 | 1.16 | 0.002954 |
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Target: 5'- cGCUGUUCGUCCCGUGAGUACAGCUCCa -3' miRNA: 3'- -CGACAAGCAGGGCACUCAUGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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