Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33619 | 3' | -47.1 | NC_007605.1 | + | 58827 | 0.66 | 0.999796 |
Target: 5'- -aGCUGggCAcguccgucAACUGGGCCAUcUCg -3' miRNA: 3'- uaCGACaaGUau------UUGACCUGGUGaAG- -5' |
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33619 | 3' | -47.1 | NC_007605.1 | + | 21042 | 0.67 | 0.999467 |
Target: 5'- -gGCUGUUCucUGAGCaUGGACCcCggUCu -3' miRNA: 3'- uaCGACAAGu-AUUUG-ACCUGGuGa-AG- -5' |
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33619 | 3' | -47.1 | NC_007605.1 | + | 134944 | 0.67 | 0.999289 |
Target: 5'- gGUGCUGUcgggCGUAGaggggggacaugugGCUGGACCAa--- -3' miRNA: 3'- -UACGACAa---GUAUU--------------UGACCUGGUgaag -5' |
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33619 | 3' | -47.1 | NC_007605.1 | + | 130876 | 0.67 | 0.999173 |
Target: 5'- gAUGCUGUUCuUAuacAGCUucucGGccacACCACUUCg -3' miRNA: 3'- -UACGACAAGuAU---UUGA----CC----UGGUGAAG- -5' |
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33619 | 3' | -47.1 | NC_007605.1 | + | 8903 | 0.68 | 0.998476 |
Target: 5'- -cGCUcUUCuuUAGGCUgagGGACCGCUUCc -3' miRNA: 3'- uaCGAcAAGu-AUUUGA---CCUGGUGAAG- -5' |
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33619 | 3' | -47.1 | NC_007605.1 | + | 70798 | 0.69 | 0.997335 |
Target: 5'- -cGCaGUaCGUGGGCUGGACCcggcuCUUCc -3' miRNA: 3'- uaCGaCAaGUAUUUGACCUGGu----GAAG- -5' |
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33619 | 3' | -47.1 | NC_007605.1 | + | 162408 | 1.1 | 0.017323 |
Target: 5'- cAUGCUGUUCAUAAACUGGACCACUUCg -3' miRNA: 3'- -UACGACAAGUAUUUGACCUGGUGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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