miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33619 3' -47.1 NC_007605.1 + 58827 0.66 0.999796
Target:  5'- -aGCUGggCAcguccgucAACUGGGCCAUcUCg -3'
miRNA:   3'- uaCGACaaGUau------UUGACCUGGUGaAG- -5'
33619 3' -47.1 NC_007605.1 + 21042 0.67 0.999467
Target:  5'- -gGCUGUUCucUGAGCaUGGACCcCggUCu -3'
miRNA:   3'- uaCGACAAGu-AUUUG-ACCUGGuGa-AG- -5'
33619 3' -47.1 NC_007605.1 + 134944 0.67 0.999289
Target:  5'- gGUGCUGUcgggCGUAGaggggggacaugugGCUGGACCAa--- -3'
miRNA:   3'- -UACGACAa---GUAUU--------------UGACCUGGUgaag -5'
33619 3' -47.1 NC_007605.1 + 130876 0.67 0.999173
Target:  5'- gAUGCUGUUCuUAuacAGCUucucGGccacACCACUUCg -3'
miRNA:   3'- -UACGACAAGuAU---UUGA----CC----UGGUGAAG- -5'
33619 3' -47.1 NC_007605.1 + 8903 0.68 0.998476
Target:  5'- -cGCUcUUCuuUAGGCUgagGGACCGCUUCc -3'
miRNA:   3'- uaCGAcAAGu-AUUUGA---CCUGGUGAAG- -5'
33619 3' -47.1 NC_007605.1 + 70798 0.69 0.997335
Target:  5'- -cGCaGUaCGUGGGCUGGACCcggcuCUUCc -3'
miRNA:   3'- uaCGaCAaGUAUUUGACCUGGu----GAAG- -5'
33619 3' -47.1 NC_007605.1 + 162408 1.1 0.017323
Target:  5'- cAUGCUGUUCAUAAACUGGACCACUUCg -3'
miRNA:   3'- -UACGACAAGUAUUUGACCUGGUGAAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.