miRNA: 3'- -UGCa--UAGUCCGAGGaCAUGuUGGuGU- -5'
miRNA: 3'- -UGCAUAGUCCGAGG-ACAUGuuGg--UGU- -5'
miRNA: 3'- ugcauaGUCCGAGGACAUGUUGgUGU- -5'
miRNA: 3'- -UGCAUAGUCCGAGGAcAUGu-------UGGUGU- -5'
miRNA: 3'- -UGCAUAGUCCGAGGACAUGUUGGUGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33637 | 5' | -51.3 | NC_007610.1 | + | 90700 | 0.66 | 0.985756 |
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Target: 5'- cACGgucAUCAGGUUCC-GUGCuACC-CAa -3' miRNA: 3'- -UGCa--UAGUCCGAGGaCAUGuUGGuGU- -5' |
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| 33637 | 5' | -51.3 | NC_007610.1 | + | 122184 | 0.66 | 0.979644 |
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Target: 5'- gACGUAUCAGGUUCUaUGUAUggUgggGCAa -3' miRNA: 3'- -UGCAUAGUCCGAGG-ACAUGuuGg--UGU- -5' |
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| 33637 | 5' | -51.3 | NC_007610.1 | + | 71654 | 0.75 | 0.658966 |
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Target: 5'- -----aCAGGCUCCUGUACAGCaACAn -3' miRNA: 3'- ugcauaGUCCGAGGACAUGUUGgUGU- -5' |
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| 33637 | 5' | -51.3 | NC_007610.1 | + | 4116 | 0.78 | 0.480253 |
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Target: 5'- gACGUAUUGGGCUCCUaUACuguaaccuGCCACAg -3' miRNA: 3'- -UGCAUAGUCCGAGGAcAUGu-------UGGUGU- -5' |
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| 33637 | 5' | -51.3 | NC_007610.1 | + | 71675 | 1.09 | 0.006244 |
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Target: 5'- aACGUAUCAGGCUCCUGUACAACCACAg -3' miRNA: 3'- -UGCAUAGUCCGAGGACAUGUUGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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