Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3370 | 5' | -49.5 | NC_001559.1 | + | 79090 | 0.66 | 0.998519 |
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Target: 5'- -gAUCCGCC--UCCGGGAguacCCAcUGAUg -3' miRNA: 3'- uaUAGGUGGuuAGGCUCU----GGUaACUA- -5' |
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| 3370 | 5' | -49.5 | NC_001559.1 | + | 9598 | 0.66 | 0.997856 |
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Target: 5'- ----aCGCCuAAUCCGAGACCAg---- -3' miRNA: 3'- uauagGUGG-UUAGGCUCUGGUaacua -5' |
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| 3370 | 5' | -49.5 | NC_001559.1 | + | 182140 | 0.66 | 0.997856 |
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Target: 5'- ----aCGCCuAAUCCGAGACCAg---- -3' miRNA: 3'- uauagGUGG-UUAGGCUCUGGUaacua -5' |
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| 3370 | 5' | -49.5 | NC_001559.1 | + | 59762 | 0.67 | 0.996962 |
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Target: 5'- --cUCCAuUUAAUgCGAGGCCGUUGGg -3' miRNA: 3'- uauAGGU-GGUUAgGCUCUGGUAACUa -5' |
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| 3370 | 5' | -49.5 | NC_001559.1 | + | 182321 | 1.03 | 0.028058 |
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Target: 5'- uAUAUCCACCAAUCCGAGACCAUUGAUu -3' miRNA: 3'- -UAUAGGUGGUUAGGCUCUGGUAACUA- -5' |
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| 3370 | 5' | -49.5 | NC_001559.1 | + | 9417 | 1.03 | 0.028058 |
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Target: 5'- uAUAUCCACCAAUCCGAGACCAUUGAUu -3' miRNA: 3'- -UAUAGGUGGUUAGGCUCUGGUAACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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