Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3374 | 3' | -40.9 | NC_001559.1 | + | 186285 | 0.66 | 1 |
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Target: 5'- aGUcgCUAccuuauuAAGAACGGAGAAgUAUCc- -3' miRNA: 3'- aCAaaGAU-------UUCUUGCCUCUU-AUAGau -5' |
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| 3374 | 3' | -40.9 | NC_001559.1 | + | 46380 | 0.67 | 1 |
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Target: 5'- cGUcaUUCUAAAaGACGGAGGAUuuccAUCa- -3' miRNA: 3'- aCA--AAGAUUUcUUGCCUCUUA----UAGau -5' |
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| 3374 | 3' | -40.9 | NC_001559.1 | + | 131340 | 0.67 | 1 |
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Target: 5'- --aUUCUuugguAGAAUGGGGAAcAUCUAc -3' miRNA: 3'- acaAAGAuu---UCUUGCCUCUUaUAGAU- -5' |
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| 3374 | 3' | -40.9 | NC_001559.1 | + | 5453 | 0.66 | 1 |
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Target: 5'- aGUcgCUAccuuauuAAGAACGGAGAAgUAUCc- -3' miRNA: 3'- aCAaaGAU-------UUCUUGCCUCUU-AUAGau -5' |
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| 3374 | 3' | -40.9 | NC_001559.1 | + | 117578 | 0.66 | 1 |
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Target: 5'- ---aUCgaAAAGAACGGAGAAUuguuGUCg- -3' miRNA: 3'- acaaAGa-UUUCUUGCCUCUUA----UAGau -5' |
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| 3374 | 3' | -40.9 | NC_001559.1 | + | 57365 | 0.77 | 0.997999 |
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Target: 5'- aUGUUgUCUGAAGGACGGAuGAAU-UCUAa -3' miRNA: 3'- -ACAA-AGAUUUCUUGCCU-CUUAuAGAU- -5' |
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| 3374 | 3' | -40.9 | NC_001559.1 | + | 57108 | 1.09 | 0.108437 |
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Target: 5'- cUGUUUCUAAAGAACGGAGAAUAUCUAu -3' miRNA: 3'- -ACAAAGAUUUCUUGCCUCUUAUAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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