Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 340 | 5' | -56 | AC_000010.1 | + | 23548 | 0.66 | 0.548117 |
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Target: 5'- cCCCCGAagaGGAacacCUACCG-CCgUGUGGGGu -3' miRNA: 3'- -GGGGCU---CCU----GAUGGUgGGgAUACUCU- -5' |
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| 340 | 5' | -56 | AC_000010.1 | + | 23393 | 0.68 | 0.473632 |
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Target: 5'- -gCgGAGGGCUGCUGCUCCgcuccgGAGAc -3' miRNA: 3'- ggGgCUCCUGAUGGUGGGGaua---CUCU- -5' |
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| 340 | 5' | -56 | AC_000010.1 | + | 23795 | 0.69 | 0.376987 |
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Target: 5'- cCCUCGAGGAUgaggaggucgACgCACCCCag-GAGAu -3' miRNA: 3'- -GGGGCUCCUGa---------UG-GUGGGGauaCUCU- -5' |
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| 340 | 5' | -56 | AC_000010.1 | + | 26277 | 0.7 | 0.368077 |
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Target: 5'- cCCCCGccAGGACUugUAUCCCUGc---- -3' miRNA: 3'- -GGGGC--UCCUGAugGUGGGGAUacucu -5' |
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| 340 | 5' | -56 | AC_000010.1 | + | 26858 | 0.71 | 0.294536 |
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Target: 5'- cUCCC-AGGACUAcuCCACCCgUAUGAa- -3' miRNA: 3'- -GGGGcUCCUGAU--GGUGGGgAUACUcu -5' |
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| 340 | 5' | -56 | AC_000010.1 | + | 25644 | 1.11 | 0.000415 |
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Target: 5'- cCCCCGAGGACUACCACCCCUAUGAGAu -3' miRNA: 3'- -GGGGCUCCUGAUGGUGGGGAUACUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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