miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
341 3' -52.6 AC_000010.1 + 26105 0.66 0.74567
Target:  5'- gAGGAcaACUGUUUGGc---GGCGGuuGCu -3'
miRNA:   3'- -UCCU--UGACGAACUauacCUGCCggCG- -5'
341 3' -52.6 AC_000010.1 + 7936 0.66 0.712165
Target:  5'- -cGAGCauucaUGCUUGugcuUGUacaGACGGCCGCa -3'
miRNA:   3'- ucCUUG-----ACGAACu---AUAc--CUGCCGGCG- -5'
341 3' -52.6 AC_000010.1 + 23813 0.7 0.495133
Target:  5'- cGGGACUGggUGGUG-GGGCGGUuaaGCu -3'
miRNA:   3'- uCCUUGACgaACUAUaCCUGCCGg--CG- -5'
341 3' -52.6 AC_000010.1 + 12134 0.71 0.452479
Target:  5'- cAGGAggugGCUGUUgcUGcaucuaacaaaaAUAUGGAUGGUCGCg -3'
miRNA:   3'- -UCCU----UGACGA--AC------------UAUACCUGCCGGCG- -5'
341 3' -52.6 AC_000010.1 + 23589 0.72 0.402124
Target:  5'- gGGGGugUGCUcccgGUGG-CGGUCGCu -3'
miRNA:   3'- -UCCUugACGAacuaUACCuGCCGGCG- -5'
341 3' -52.6 AC_000010.1 + 30925 1.03 0.002716
Target:  5'- gAGGAACUGCUUGA-AUGGACGGCCGCa -3'
miRNA:   3'- -UCCUUGACGAACUaUACCUGCCGGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.