Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 344 | 3' | -56.5 | AC_000011.1 | + | 32406 | 0.66 | 0.558265 |
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Target: 5'- aGAGGGGcggcggccuuGGugGCCgUGUUggAGAUGAGu -3' miRNA: 3'- -CUCCCC----------UCugCGGgGCAG--UCUAUUCc -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 4988 | 0.66 | 0.514963 |
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Target: 5'- uGGGGGGAGAgCGCCUCcuggCGGAacuGGu -3' miRNA: 3'- -CUCCCCUCU-GCGGGGca--GUCUauuCC- -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 6710 | 0.67 | 0.483399 |
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Target: 5'- cGAGGGGGcGAggaGCCCCGggccCAGGUu-GGu -3' miRNA: 3'- -CUCCCCU-CUg--CGGGGCa---GUCUAuuCC- -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 4843 | 0.68 | 0.432992 |
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Target: 5'- uGAGGGaGAGACaGCUgCCGUCcucccGGAgGAGGg -3' miRNA: 3'- -CUCCC-CUCUG-CGG-GGCAG-----UCUaUUCC- -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 17381 | 0.68 | 0.432992 |
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Target: 5'- -cGGGGAu-CGCCCCGaUCGccgcggcgauGAUGGGGa -3' miRNA: 3'- cuCCCCUcuGCGGGGC-AGU----------CUAUUCC- -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 9444 | 0.68 | 0.404256 |
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Target: 5'- cGGGGGGGu--CCCCGUUGGGcAGGGa -3' miRNA: 3'- cUCCCCUCugcGGGGCAGUCUaUUCC- -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 4693 | 0.69 | 0.394948 |
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Target: 5'- uGAGGGGAacuACGCCCCcg-GGAUcGAGGg -3' miRNA: 3'- -CUCCCCUc--UGCGGGGcagUCUA-UUCC- -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 26884 | 0.7 | 0.342096 |
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Target: 5'- -cGGGGAGAucuuccuucaCGCCUCGUCAGGc---- -3' miRNA: 3'- cuCCCCUCU----------GCGGGGCAGUCUauucc -5' |
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| 344 | 3' | -56.5 | AC_000011.1 | + | 3586 | 1.11 | 0.000342 |
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Target: 5'- aGAGGGGAGACGCCCCGUCAGAUAAGGg -3' miRNA: 3'- -CUCCCCUCUGCGGGGCAGUCUAUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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