Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
344 | 5' | -57.8 | AC_000011.1 | + | 15599 | 0.66 | 0.495374 |
Target: 5'- uCCCUggggcgCCCUCAAGGGccgcgugcGGUCGCgcaCCa -3' miRNA: 3'- -GGGGa-----GGGAGUUUCC--------UCGGCGaa-GGc -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 2135 | 0.66 | 0.441768 |
Target: 5'- aCCCUCCggCGGAGGAGgaggaguagcugacCUGUUUCCu -3' miRNA: 3'- gGGGAGGgaGUUUCCUC--------------GGCGAAGGc -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 17970 | 0.67 | 0.397092 |
Target: 5'- aCCgCCUCCUcgUAcGGGGGCgGCUcgUCCGu -3' miRNA: 3'- -GG-GGAGGGa-GUuUCCUCGgCGA--AGGC- -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 23282 | 0.67 | 0.387968 |
Target: 5'- gCCCCUUCCgCGuucGGGGGUgCGCUcCCGg -3' miRNA: 3'- -GGGGAGGGaGUu--UCCUCG-GCGAaGGC- -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 17867 | 0.68 | 0.370138 |
Target: 5'- aCCCCgcCCCgaugCGGAGGAGaCGCUgCUGa -3' miRNA: 3'- -GGGGa-GGGa---GUUUCCUCgGCGAaGGC- -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 17924 | 0.68 | 0.370138 |
Target: 5'- uCUCCUCCgcaUCGGGgcGGGGUCGCUUCUc -3' miRNA: 3'- -GGGGAGGg--AGUUU--CCUCGGCGAAGGc -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 7161 | 0.68 | 0.361435 |
Target: 5'- gCCCUCCCcguggaGAAGG-GCUGCUgcgCCu -3' miRNA: 3'- gGGGAGGGag----UUUCCuCGGCGAa--GGc -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 8549 | 0.69 | 0.328068 |
Target: 5'- gCCCCUgCCUCcGGGccccGAGCCGCcccugCCGc -3' miRNA: 3'- -GGGGAgGGAGuUUC----CUCGGCGaa---GGC- -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 8767 | 0.69 | 0.297035 |
Target: 5'- uCUCCUCCuCUUGAAGGucuCCGCggCCGg -3' miRNA: 3'- -GGGGAGG-GAGUUUCCuc-GGCGaaGGC- -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 20237 | 0.71 | 0.223538 |
Target: 5'- uCCCCUCgCgCAAcuGGGCCGcCUUCCGc -3' miRNA: 3'- -GGGGAGgGaGUUucCUCGGC-GAAGGC- -5' |
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344 | 5' | -57.8 | AC_000011.1 | + | 3552 | 1.1 | 0.000305 |
Target: 5'- aCCCCUCCCUCAAAGGAGCCGCUUCCGc -3' miRNA: 3'- -GGGGAGGGAGUUUCCUCGGCGAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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