Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3446 | 5' | -52.6 | NC_001604.1 | + | 1338 | 0.67 | 0.76584 |
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Target: 5'- uGGUGCGCugUGGcuuuaacuGGUCACUCGcaAUGGc -3' miRNA: 3'- -CUAUGCGugGCU--------UCAGUGAGCc-UGCC- -5' |
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| 3446 | 5' | -52.6 | NC_001604.1 | + | 28265 | 0.67 | 0.755308 |
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Target: 5'- --aACGCACCGGAugcguaagCACUCGcaGCGGa -3' miRNA: 3'- cuaUGCGUGGCUUca------GUGAGCc-UGCC- -5' |
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| 3446 | 5' | -52.6 | NC_001604.1 | + | 15700 | 0.67 | 0.733856 |
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Target: 5'- -uUugGCGCUGAGcacCAUUUGGAUGGg -3' miRNA: 3'- cuAugCGUGGCUUca-GUGAGCCUGCC- -5' |
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| 3446 | 5' | -52.6 | NC_001604.1 | + | 18967 | 0.68 | 0.678554 |
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Target: 5'- gGAUACcgaggGCACCGAAGUguCU-GGACGc -3' miRNA: 3'- -CUAUG-----CGUGGCUUCAguGAgCCUGCc -5' |
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| 3446 | 5' | -52.6 | NC_001604.1 | + | 14467 | 0.73 | 0.380868 |
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Target: 5'- gGAUGCGCuggaaGCCGAGGUUGCaCGaGGCGGu -3' miRNA: 3'- -CUAUGCG-----UGGCUUCAGUGaGC-CUGCC- -5' |
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| 3446 | 5' | -52.6 | NC_001604.1 | + | 14505 | 1.11 | 0.001186 |
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Target: 5'- aGAUACGCACCGAAGUCACUCGGACGGu -3' miRNA: 3'- -CUAUGCGUGGCUUCAGUGAGCCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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