Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
345 | 3' | -63.6 | AC_000011.1 | + | 3353 | 0.66 | 0.254313 |
Target: 5'- cCCugCaCCGGGACGGaGUUCGguUCCAg -3' miRNA: 3'- -GGugGgGGCCCUGCC-CGAGUacGGGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 9445 | 0.66 | 0.248027 |
Target: 5'- gCgGCCCCgCGaGGACGGGCgc--GCCg- -3' miRNA: 3'- -GgUGGGG-GC-CCUGCCCGaguaCGGgu -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 15645 | 0.66 | 0.24187 |
Target: 5'- gCGCCCCaGGGAgCGcGGaa-GUGCCCGc -3' miRNA: 3'- gGUGGGGgCCCU-GC-CCgagUACGGGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 25240 | 0.66 | 0.22359 |
Target: 5'- cUCACCCCgGGGcuguggaccuCGGccuacuugcgcaaGUUCGUGCCCGa -3' miRNA: 3'- -GGUGGGGgCCCu---------GCC-------------CGAGUACGGGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 8895 | 0.67 | 0.192042 |
Target: 5'- aCGCCCUCGGGAUcgccGGCgcgCAUGaCCAc -3' miRNA: 3'- gGUGGGGGCCCUGc---CCGa--GUACgGGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 4682 | 0.69 | 0.139747 |
Target: 5'- aCGCCCCCGGGAUcgaGGGUaCcuuUGUCCc -3' miRNA: 3'- gGUGGGGGCCCUG---CCCGaGu--ACGGGu -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 24495 | 0.69 | 0.136035 |
Target: 5'- aCUACCUCUucaGGcACGGGUUCGUGCgCCAg -3' miRNA: 3'- -GGUGGGGG---CCcUGCCCGAGUACG-GGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 6358 | 0.7 | 0.132056 |
Target: 5'- aUCACCCUgcauaauCGGGGCuGGCaggucagaaUCGUGCCCGa -3' miRNA: 3'- -GGUGGGG-------GCCCUGcCCG---------AGUACGGGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 18137 | 0.7 | 0.128881 |
Target: 5'- -uGCCCCCGGGucgcgcGCGGGC-CAcgGCCa- -3' miRNA: 3'- ggUGGGGGCCC------UGCCCGaGUa-CGGgu -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 23340 | 0.7 | 0.125436 |
Target: 5'- gCACCCCCGaacgcGGAagGGGCUC-UGCCa- -3' miRNA: 3'- gGUGGGGGC-----CCUg-CCCGAGuACGGgu -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 19881 | 0.73 | 0.07431 |
Target: 5'- -uGCUCCUGGGcaACGGGCgcuaCGUGCCCu -3' miRNA: 3'- ggUGGGGGCCC--UGCCCGa---GUACGGGu -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 21613 | 0.74 | 0.059214 |
Target: 5'- gCACCCCCGucagcaaguucauGGugGGGUUCuUGUCCAu -3' miRNA: 3'- gGUGGGGGC-------------CCugCCCGAGuACGGGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 13187 | 0.76 | 0.043387 |
Target: 5'- cCCGCCgCCGGGguucuacACGGGCgaguacgaCAUGCCCGa -3' miRNA: 3'- -GGUGGgGGCCC-------UGCCCGa-------GUACGGGU- -5' |
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345 | 3' | -63.6 | AC_000011.1 | + | 4143 | 1.09 | 0.000106 |
Target: 5'- uCCACCCCCGGGACGGGCUCAUGCCCAu -3' miRNA: 3'- -GGUGGGGGCCCUGCCCGAGUACGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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