Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 345 | 5' | -58.8 | AC_000011.1 | + | 10738 | 0.66 | 0.419994 |
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Target: 5'- aUCC-GGCCgGCCUCgaACCGGGgcacaCCg- -3' miRNA: 3'- -AGGuUCGGgUGGAGa-UGGCCCa----GGac -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 11204 | 0.67 | 0.351446 |
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Target: 5'- gUCCAGGCCgCGCCgcagCUcccgccccgcguggaACCGGG-CCg- -3' miRNA: 3'- -AGGUUCGG-GUGGa---GA---------------UGGCCCaGGac -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 2641 | 0.68 | 0.307784 |
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Target: 5'- aUCCAuGCCCACCaCUcCCGGGUa--- -3' miRNA: 3'- -AGGUuCGGGUGGaGAuGGCCCAggac -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 12332 | 0.69 | 0.250919 |
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Target: 5'- aCCAGGCCgGCCUCgucGCCGcGGauggCCUu -3' miRNA: 3'- aGGUUCGGgUGGAGa--UGGC-CCa---GGAc -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 14797 | 0.7 | 0.214182 |
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Target: 5'- gCCGuAGCuaCCGCCUCUACCGaGGUCa-- -3' miRNA: 3'- aGGU-UCG--GGUGGAGAUGGC-CCAGgac -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 11995 | 0.7 | 0.214182 |
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Target: 5'- gUCCAccucaucGUCCACCUCUucuACguaGGGUCCUGc -3' miRNA: 3'- -AGGUu------CGGGUGGAGA---UGg--CCCAGGAC- -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 19943 | 0.72 | 0.158783 |
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Target: 5'- aUCAAGagccuCCugCUCUGcCCGGGUCCUa -3' miRNA: 3'- aGGUUCg----GGugGAGAU-GGCCCAGGAc -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 933 | 0.74 | 0.117982 |
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Target: 5'- cUUUAAGCUcggggaucuuuuucuCACCUCUGCCGGGUCUa- -3' miRNA: 3'- -AGGUUCGG---------------GUGGAGAUGGCCCAGGac -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 15975 | 0.74 | 0.116652 |
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Target: 5'- gCCGuGGCCgCGCgUCU-CCGGGUCCUGc -3' miRNA: 3'- aGGU-UCGG-GUGgAGAuGGCCCAGGAC- -5' |
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| 345 | 5' | -58.8 | AC_000011.1 | + | 4105 | 1.09 | 0.000239 |
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Target: 5'- aUCCAAGCCCACCUCUACCGGGUCCUGg -3' miRNA: 3'- -AGGUUCGGGUGGAGAUGGCCCAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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