Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3451 | 5' | -42.1 | NC_001604.1 | + | 19468 | 0.66 | 0.999875 |
Target: 5'- ---aAGCAUGAAGGCGAgacaauGCGuguauacuuuaaGGAGGUa -3' miRNA: 3'- caagUUGUAUUUCCGUU------UGC------------UUUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 39181 | 0.66 | 0.999875 |
Target: 5'- -cUCAcuAUAgggAGAGGagGGACGAAAGGUu -3' miRNA: 3'- caAGU--UGUa--UUUCCg-UUUGCUUUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 19369 | 0.66 | 0.999875 |
Target: 5'- -aUUAAUccuGAGGCAGACucuAAGGGCu -3' miRNA: 3'- caAGUUGuauUUCCGUUUGc--UUUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 22732 | 0.66 | 0.999875 |
Target: 5'- ---aAGCGUGAAGGCuuuGCGGAccGUa -3' miRNA: 3'- caagUUGUAUUUCCGuu-UGCUUucCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 34327 | 0.66 | 0.999775 |
Target: 5'- --cUAACGUAGGGGCuaccuuAAUGAAugcugcuGGCg -3' miRNA: 3'- caaGUUGUAUUUCCGu-----UUGCUUu------CCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 17979 | 0.66 | 0.999702 |
Target: 5'- ---gAGCAccGAAGGCAGACGGAuuGGa -3' miRNA: 3'- caagUUGUa-UUUCCGUUUGCUUu-CCg -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 20616 | 0.66 | 0.999609 |
Target: 5'- aGUUCAugAUGAuacGCucuaccauCGAGAGGCc -3' miRNA: 3'- -CAAGUugUAUUuc-CGuuu-----GCUUUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 26645 | 0.67 | 0.999492 |
Target: 5'- cUUCAugucCAUAAAGGC--ACGAuAGGg -3' miRNA: 3'- cAAGUu---GUAUUUCCGuuUGCUuUCCg -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 13385 | 0.67 | 0.999492 |
Target: 5'- --aCAACGUGGAaGcCAAGCGAcugaacgauGAGGCu -3' miRNA: 3'- caaGUUGUAUUUcC-GUUUGCU---------UUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 7956 | 0.67 | 0.999345 |
Target: 5'- cGUUCAAuCGUAuguGCGAGCGAGcgccGGCu -3' miRNA: 3'- -CAAGUU-GUAUuucCGUUUGCUUu---CCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 1902 | 0.67 | 0.99894 |
Target: 5'- aGggCGGCGU---GGCAuaGACGAAAGGa -3' miRNA: 3'- -CaaGUUGUAuuuCCGU--UUGCUUUCCg -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 21301 | 0.69 | 0.996255 |
Target: 5'- gGUUCAGCGUAcAGGUGAACGuguGAccGCc -3' miRNA: 3'- -CAAGUUGUAUuUCCGUUUGC---UUucCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 26140 | 0.69 | 0.995403 |
Target: 5'- aGUUUGACGUAcAGGacCGAGCGAGaccuuuugggauuGGGCg -3' miRNA: 3'- -CAAGUUGUAUuUCC--GUUUGCUU-------------UCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 12520 | 0.7 | 0.990747 |
Target: 5'- -gUCAACAagcucuacaauGGGGCAcAGCGAuGGGCa -3' miRNA: 3'- caAGUUGUau---------UUCCGU-UUGCUuUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 17518 | 0.71 | 0.987786 |
Target: 5'- aGUU--ACGUGAAGGCugcGACGAcaAGGGUa -3' miRNA: 3'- -CAAguUGUAUUUCCGu--UUGCU--UUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 1082 | 0.73 | 0.951784 |
Target: 5'- cUUUAGCGUAAuGGCAAGUG-AGGGCa -3' miRNA: 3'- cAAGUUGUAUUuCCGUUUGCuUUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 35587 | 0.79 | 0.75055 |
Target: 5'- uGUUCAACGUAGAGaGUGgacaacAGCGAuAGGCg -3' miRNA: 3'- -CAAGUUGUAUUUC-CGU------UUGCUuUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 11150 | 0.8 | 0.702442 |
Target: 5'- --cCAACGcuuAAGGuCGAACGAAGGGCa -3' miRNA: 3'- caaGUUGUau-UUCC-GUUUGCUUUCCG- -5' |
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3451 | 5' | -42.1 | NC_001604.1 | + | 21335 | 1.16 | 0.006256 |
Target: 5'- aGUUCAACAUAAAGGCAAACGAAAGGCg -3' miRNA: 3'- -CAAGUUGUAUUUCCGUUUGCUUUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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