Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3452 | 5' | -55.2 | NC_001604.1 | + | 28833 | 0.66 | 0.609939 |
Target: 5'- uGguGCgCAUGauaacuUCUUGAGCCAGCa-AGCu -3' miRNA: 3'- -CguCG-GUAU------GGAACUCGGUCGcgUCG- -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 18898 | 0.66 | 0.598552 |
Target: 5'- -gAGCCGU----UGAGCCAGCGCc-- -3' miRNA: 3'- cgUCGGUAuggaACUCGGUCGCGucg -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 29762 | 0.66 | 0.598552 |
Target: 5'- gGCAGaCCAc-UCUUGA-CCAGCGuCAGUg -3' miRNA: 3'- -CGUC-GGUauGGAACUcGGUCGC-GUCG- -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 34064 | 0.66 | 0.595141 |
Target: 5'- uGCAGCCAUagcauagaaACCaccagcgagacccaUagaggugaUGAuGCuCAGCGCAGCg -3' miRNA: 3'- -CGUCGGUA---------UGG--------------A--------ACU-CG-GUCGCGUCG- -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 5068 | 0.66 | 0.587195 |
Target: 5'- cCAGCCAUugACC---AGCCAGUGCcuuAGUg -3' miRNA: 3'- cGUCGGUA--UGGaacUCGGUCGCG---UCG- -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 29310 | 0.66 | 0.575879 |
Target: 5'- -aAGCUGU-CCUUGGGUccucuugguuCAGCGCAGa -3' miRNA: 3'- cgUCGGUAuGGAACUCG----------GUCGCGUCg -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 21633 | 0.69 | 0.426165 |
Target: 5'- -gGGUCGUcCCgcauagGuGCCAGUGCAGCc -3' miRNA: 3'- cgUCGGUAuGGaa----CuCGGUCGCGUCG- -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 38703 | 0.69 | 0.406655 |
Target: 5'- --uGCgAUAcCCUUGAGCCAGUcauGCAGa -3' miRNA: 3'- cguCGgUAU-GGAACUCGGUCG---CGUCg -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 3078 | 0.7 | 0.361304 |
Target: 5'- aUAGCCAU-CCggucagcauuacGCCAGUGCAGCg -3' miRNA: 3'- cGUCGGUAuGGaacu--------CGGUCGCGUCG- -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 15847 | 0.75 | 0.180781 |
Target: 5'- aGCgAGCCAUGaagUGAGCCaagcAGCGUAGCu -3' miRNA: 3'- -CG-UCGGUAUggaACUCGG----UCGCGUCG- -5' |
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3452 | 5' | -55.2 | NC_001604.1 | + | 21799 | 1.14 | 0.000229 |
Target: 5'- uGCAGCCAUACCUUGAGCCAGCGCAGCa -3' miRNA: 3'- -CGUCGGUAUGGAACUCGGUCGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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