Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3453 | 5' | -48.2 | NC_001604.1 | + | 5229 | 0.67 | 0.891184 |
Target: 5'- uCCAGCAGCCUga--UUCGgcuGAGUGa- -3' miRNA: 3'- -GGUCGUCGGAaucgAAGUu--CUUACag -5' |
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3453 | 5' | -48.2 | NC_001604.1 | + | 38253 | 0.68 | 0.88315 |
Target: 5'- gUAGCGGCCUUAGaCUUaGAGAAg--- -3' miRNA: 3'- gGUCGUCGGAAUC-GAAgUUCUUacag -5' |
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3453 | 5' | -48.2 | NC_001604.1 | + | 21834 | 0.69 | 0.798172 |
Target: 5'- gCAGCAGCCaUAGCcUCAGGugaagcUGUa -3' miRNA: 3'- gGUCGUCGGaAUCGaAGUUCuu----ACAg -5' |
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3453 | 5' | -48.2 | NC_001604.1 | + | 29668 | 0.71 | 0.720129 |
Target: 5'- aUAGCAuCCUUGGCaccgUCuGGAAUGUCc -3' miRNA: 3'- gGUCGUcGGAAUCGa---AGuUCUUACAG- -5' |
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3453 | 5' | -48.2 | NC_001604.1 | + | 27405 | 1.14 | 0.001341 |
Target: 5'- aCCAGCAGCCUUAGCUUCAAGAAUGUCa -3' miRNA: 3'- -GGUCGUCGGAAUCGAAGUUCUUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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