miRNA: 3'- gaUGuCCC--UCUUCUAAUAAaGCgGAG- -5'
miRNA: 3'- -GAUGUCCCUCUucuaa---UAAAGcGGAG- -5'
miRNA: 3'- gAUGUCCCUCUUcuaAUAaaGCGGAG- -5'
miRNA: 3'- -GAUGUCCCUCUUCUAAUAAAGCGGAG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3455 | 3' | -46.9 | NC_001604.1 | + | 23479 | 0.66 | 0.977086 |
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Target: 5'- -cGCuGGGuaAGGAGAUUAUUgCGgCUCu -3' miRNA: 3'- gaUGuCCC--UCUUCUAAUAAaGCgGAG- -5' |
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| 3455 | 3' | -46.9 | NC_001604.1 | + | 22876 | 0.68 | 0.933008 |
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Target: 5'- aCUAUAGGGAGAccacaacgGUUUC-CCUCu -3' miRNA: 3'- -GAUGUCCCUCUucuaa---UAAAGcGGAG- -5' |
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| 3455 | 3' | -46.9 | NC_001604.1 | + | 26568 | 0.69 | 0.908068 |
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Target: 5'- uUACAGGGAGAAcccUAUcgUGCCUUu -3' miRNA: 3'- gAUGUCCCUCUUcuaAUAaaGCGGAG- -5' |
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| 3455 | 3' | -46.9 | NC_001604.1 | + | 34601 | 1.12 | 0.00318 |
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Target: 5'- aCUACAGGGAGAAGAUUAUUUCGCCUCu -3' miRNA: 3'- -GAUGUCCCUCUUCUAAUAAAGCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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