Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3457 | 5' | -51.1 | NC_001604.1 | + | 33253 | 0.67 | 0.766148 |
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Target: 5'- ---aGGCGuUCUUAGCGAaGAACCCuAGg -3' miRNA: 3'- cuuaUCGU-AGGGUCGCU-CUUGGGuUC- -5' |
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| 3457 | 5' | -51.1 | NC_001604.1 | + | 32376 | 0.67 | 0.744178 |
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Target: 5'- --uUAGCGcCgCCAGUaGAGAACUCAGGg -3' miRNA: 3'- cuuAUCGUaG-GGUCG-CUCUUGGGUUC- -5' |
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| 3457 | 5' | -51.1 | NC_001604.1 | + | 15808 | 0.69 | 0.628794 |
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Target: 5'- cGGAU-GCGUCgaugCCAGCcuGAACCCAAGg -3' miRNA: 3'- -CUUAuCGUAG----GGUCGcuCUUGGGUUC- -5' |
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| 3457 | 5' | -51.1 | NC_001604.1 | + | 23163 | 0.7 | 0.570409 |
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Target: 5'- --cUGGC-UCCgGGCGAGAACCuCGAc -3' miRNA: 3'- cuuAUCGuAGGgUCGCUCUUGG-GUUc -5' |
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| 3457 | 5' | -51.1 | NC_001604.1 | + | 34058 | 0.71 | 0.52466 |
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Target: 5'- -cAUAGCAUagaaaccaCCAGCGAG-ACCCAu- -3' miRNA: 3'- cuUAUCGUAg-------GGUCGCUCuUGGGUuc -5' |
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| 3457 | 5' | -51.1 | NC_001604.1 | + | 36973 | 1.1 | 0.001222 |
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Target: 5'- cGAAUAGCAUCCCAGCGAGAACCCAAGg -3' miRNA: 3'- -CUUAUCGUAGGGUCGCUCUUGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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