Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3458 | 3' | -48.8 | NC_001604.1 | + | 17791 | 0.66 | 0.945545 |
Target: 5'- aCCguAAGGCcgugaagAAACCUGuAGGGUACUUUg -3' miRNA: 3'- -GGguUUCUG-------UUUGGGU-UCCCAUGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 29257 | 0.66 | 0.940892 |
Target: 5'- aCCCAAGGAC-AGCUUggGaGaUGCUUCa -3' miRNA: 3'- -GGGUUUCUGuUUGGGuuC-CcAUGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 39467 | 0.66 | 0.935429 |
Target: 5'- aCCCAAGGGCAGcacaaaguACCaCAAGaaaucgguaGGUgACUCUa -3' miRNA: 3'- -GGGUUUCUGUU--------UGG-GUUC---------CCA-UGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 22224 | 0.67 | 0.923572 |
Target: 5'- uUCCGAAGAguuuACCCcacuGGGcgagACUCCa -3' miRNA: 3'- -GGGUUUCUguu-UGGGuu--CCCa---UGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 17694 | 0.67 | 0.91047 |
Target: 5'- aCCCGuAAGA-AGGCUUggGcaGGUGCUCCa -3' miRNA: 3'- -GGGU-UUCUgUUUGGGuuC--CCAUGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 23374 | 0.67 | 0.903457 |
Target: 5'- gCCAGAGAUucACCCAacugAGaGGUauACUCa -3' miRNA: 3'- gGGUUUCUGuuUGGGU----UC-CCA--UGAGg -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 7608 | 0.68 | 0.880609 |
Target: 5'- aCCCGAAGGUGAGCCagugugauuaCAuuuucucuugAGGGUugUCCu -3' miRNA: 3'- -GGGUUUCUGUUUGG----------GU----------UCCCAugAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 19233 | 0.68 | 0.87241 |
Target: 5'- uCCUuuAGGC-AACCCAAGGuGU--UCCa -3' miRNA: 3'- -GGGuuUCUGuUUGGGUUCC-CAugAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 1424 | 0.69 | 0.836894 |
Target: 5'- gCUGAGuGAUAGACUCAAGGucGCUCCu -3' miRNA: 3'- gGGUUU-CUGUUUGGGUUCCcaUGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 19373 | 0.7 | 0.80768 |
Target: 5'- aUCCuGAGGCAGACuCUAAGGGcuuacagcgAUUCCu -3' miRNA: 3'- -GGGuUUCUGUUUG-GGUUCCCa--------UGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 36964 | 0.7 | 0.80768 |
Target: 5'- uCCCAgcGA-GAACCCAAGGGauaGCUUa -3' miRNA: 3'- -GGGUuuCUgUUUGGGUUCCCa--UGAGg -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 15397 | 0.7 | 0.787148 |
Target: 5'- aCCAAGuAC--ACCgaUAAGGGUGCUCCu -3' miRNA: 3'- gGGUUUcUGuuUGG--GUUCCCAUGAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 34046 | 0.71 | 0.721623 |
Target: 5'- gCCUAAGGAgAAACgUAAGGaGUACUUg -3' miRNA: 3'- -GGGUUUCUgUUUGgGUUCC-CAUGAGg -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 171 | 1 | 0.015861 |
Target: 5'- aCCCAAAGACAAACCCAAGGGUcaUCCc -3' miRNA: 3'- -GGGUUUCUGUUUGGGUUCCCAugAGG- -5' |
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3458 | 3' | -48.8 | NC_001604.1 | + | 39909 | 1 | 0.015861 |
Target: 5'- aCCCAAAGACAAACCCAAGGGUcaUCCc -3' miRNA: 3'- -GGGUUUCUGUUUGGGUUCCCAugAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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