Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 346 | 3' | -53.4 | AC_000011.1 | + | 7282 | 0.66 | 0.710428 |
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Target: 5'- cCAGcUCUGGGagcaGGGGGGCUGCGaCg -3' miRNA: 3'- cGUCuAGACUCa---CCCCUUGAUGCgGg -5' |
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| 346 | 3' | -53.4 | AC_000011.1 | + | 22436 | 0.67 | 0.676643 |
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Target: 5'- aGCGGuUCUGGGUGau---CUugGCCCg -3' miRNA: 3'- -CGUCuAGACUCACcccuuGAugCGGG- -5' |
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| 346 | 3' | -53.4 | AC_000011.1 | + | 31083 | 0.67 | 0.676642 |
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Target: 5'- -gGGA-CUGGGUGGGGGACcggGgGUCg -3' miRNA: 3'- cgUCUaGACUCACCCCUUGa--UgCGGg -5' |
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| 346 | 3' | -53.4 | AC_000011.1 | + | 25867 | 0.68 | 0.608155 |
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Target: 5'- --cGGUCUGGGUGGuGGggUUcgggaauCGUCCg -3' miRNA: 3'- cguCUAGACUCACC-CCuuGAu------GCGGG- -5' |
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| 346 | 3' | -53.4 | AC_000011.1 | + | 748 | 0.69 | 0.515104 |
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Target: 5'- uGCAGcgaagguGUCUcaaaugGGGUGGGGGGCUccggagggucguCGCCCa -3' miRNA: 3'- -CGUC-------UAGA------CUCACCCCUUGAu-----------GCGGG- -5' |
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| 346 | 3' | -53.4 | AC_000011.1 | + | 21363 | 0.7 | 0.464969 |
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Target: 5'- aGCAGAUCUaccaguucGAGUacGaGGGccuGCUGCGCCg -3' miRNA: 3'- -CGUCUAGA--------CUCA--C-CCCu--UGAUGCGGg -5' |
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| 346 | 3' | -53.4 | AC_000011.1 | + | 6643 | 0.7 | 0.464969 |
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Target: 5'- uGCcGAUgUaGGUGGGGuAGCaGCGCCCc -3' miRNA: 3'- -CGuCUAgAcUCACCCC-UUGaUGCGGG- -5' |
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| 346 | 3' | -53.4 | AC_000011.1 | + | 4703 | 0.97 | 0.007778 |
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Target: 5'- uGCAGAUCUGuGaGGGGAACUACGCCCc -3' miRNA: 3'- -CGUCUAGACuCaCCCCUUGAUGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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