Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3476 | 3' | -47 | NC_001609.1 | + | 6687 | 0.66 | 0.75762 |
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Target: 5'- --cGgcAGU-GACG--ACCGCCGCCGu -3' miRNA: 3'- guaCuuUUAuCUGCauUGGCGGUGGC- -5' |
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| 3476 | 3' | -47 | NC_001609.1 | + | 5581 | 0.68 | 0.634893 |
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Target: 5'- --aGAAGGcggGGGCGUGgguuuccagcguuucACCGuCCACCGg -3' miRNA: 3'- guaCUUUUa--UCUGCAU---------------UGGC-GGUGGC- -5' |
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| 3476 | 3' | -47 | NC_001609.1 | + | 5901 | 0.68 | 0.629663 |
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Target: 5'- cUAUGAAAAUGG-UGUcuggaagguGCUGCCGCCa -3' miRNA: 3'- -GUACUUUUAUCuGCAu--------UGGCGGUGGc -5' |
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| 3476 | 3' | -47 | NC_001609.1 | + | 9886 | 0.7 | 0.537826 |
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Target: 5'- -cUGAAAG-GGGCGUGugacagcacggccACCGCCuACCGg -3' miRNA: 3'- guACUUUUaUCUGCAU-------------UGGCGG-UGGC- -5' |
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| 3476 | 3' | -47 | NC_001609.1 | + | 5118 | 0.74 | 0.295763 |
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Target: 5'- uCcgGGAAGUGaauaaucacccgucGGCGUGACaCGCCGCCGc -3' miRNA: 3'- -GuaCUUUUAU--------------CUGCAUUG-GCGGUGGC- -5' |
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| 3476 | 3' | -47 | NC_001609.1 | + | 6975 | 0.96 | 0.009399 |
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Target: 5'- cCAUGAAAAUGaACGUAACCGCCACCGu -3' miRNA: 3'- -GUACUUUUAUcUGCAUUGGCGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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