Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3479 | 5' | -59 | NC_001609.1 | + | 7811 | 0.66 | 0.153187 |
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Target: 5'- cCGugGGAaauGCCGUAcCGCUGcCCGGa -3' miRNA: 3'- aGUugCCUg--UGGCGUaGCGGC-GGCCa -5' |
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| 3479 | 5' | -59 | NC_001609.1 | + | 7728 | 0.66 | 0.153187 |
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Target: 5'- -uGACGGACGCaccacaGaCAUC-CCGCUGGg -3' miRNA: 3'- agUUGCCUGUGg-----C-GUAGcGGCGGCCa -5' |
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| 3479 | 5' | -59 | NC_001609.1 | + | 7195 | 0.67 | 0.144236 |
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Target: 5'- gUCAcUGGAgGCgGcCAUCGCCGCUGa- -3' miRNA: 3'- -AGUuGCCUgUGgC-GUAGCGGCGGCca -5' |
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| 3479 | 5' | -59 | NC_001609.1 | + | 5140 | 0.67 | 0.127753 |
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Target: 5'- gUCGGCGuGACacGCCGCcgCuGCgGUCGGUg -3' miRNA: 3'- -AGUUGC-CUG--UGGCGuaG-CGgCGGCCA- -5' |
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| 3479 | 5' | -59 | NC_001609.1 | + | 8229 | 0.68 | 0.119813 |
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Target: 5'- aCGGCGGGCACUGCGUucaucacCGgCGaCGGUg -3' miRNA: 3'- aGUUGCCUGUGGCGUA-------GCgGCgGCCA- -5' |
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| 3479 | 5' | -59 | NC_001609.1 | + | 5246 | 1.08 | 5.7e-05 |
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Target: 5'- gUCAACGGACACCGCAUCGCCGCCGGUg -3' miRNA: 3'- -AGUUGCCUGUGGCGUAGCGGCGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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