Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
348 | 3' | -62 | AC_000011.1 | + | 13364 | 0.66 | 0.30006 |
Target: 5'- uGCUgCCGCGGCgGUgCCCgAGgCCGCc- -3' miRNA: 3'- -UGAgGGCGUCGgCA-GGG-UUgGGCGcu -5' |
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348 | 3' | -62 | AC_000011.1 | + | 22957 | 0.66 | 0.284919 |
Target: 5'- aGCU-CCGCuuGCCGUCCUucucgguGAUCCGCa- -3' miRNA: 3'- -UGAgGGCGu-CGGCAGGG-------UUGGGCGcu -5' |
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348 | 3' | -62 | AC_000011.1 | + | 30554 | 0.66 | 0.278617 |
Target: 5'- uCUUCUGCcGCCGcauUCCCAACgCGCa- -3' miRNA: 3'- uGAGGGCGuCGGC---AGGGUUGgGCGcu -5' |
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348 | 3' | -62 | AC_000011.1 | + | 17000 | 0.66 | 0.271745 |
Target: 5'- cACUCgCCGCcGCCGUCgCC-GCaCCGCc- -3' miRNA: 3'- -UGAG-GGCGuCGGCAG-GGuUG-GGCGcu -5' |
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348 | 3' | -62 | AC_000011.1 | + | 27136 | 0.67 | 0.258412 |
Target: 5'- cAUUCaauCGUAGCCGUCCacCGACUCGCuGAu -3' miRNA: 3'- -UGAGg--GCGUCGGCAGG--GUUGGGCG-CU- -5' |
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348 | 3' | -62 | AC_000011.1 | + | 3580 | 0.67 | 0.245618 |
Target: 5'- -aUCCaCGguggaCGGCCGgCCCGugcaGCCCGCGAa -3' miRNA: 3'- ugAGG-GC-----GUCGGCaGGGU----UGGGCGCU- -5' |
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348 | 3' | -62 | AC_000011.1 | + | 17785 | 0.67 | 0.227418 |
Target: 5'- --aCCCGguGCCG-CCCGccgGCuCCGUGGa -3' miRNA: 3'- ugaGGGCguCGGCaGGGU---UG-GGCGCU- -5' |
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348 | 3' | -62 | AC_000011.1 | + | 21180 | 0.68 | 0.194438 |
Target: 5'- aGCUCCUGCucGCCGg---AGCCCGCGGc -3' miRNA: 3'- -UGAGGGCGu-CGGCagggUUGGGCGCU- -5' |
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348 | 3' | -62 | AC_000011.1 | + | 21906 | 0.69 | 0.16572 |
Target: 5'- -gUUCCGCAaCgUGUCCCuGCCCGCGGg -3' miRNA: 3'- ugAGGGCGUcG-GCAGGGuUGGGCGCU- -5' |
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348 | 3' | -62 | AC_000011.1 | + | 22070 | 0.7 | 0.152836 |
Target: 5'- aACUCCCGCGcGCagaacgcggGUCCCAACU-GCGAu -3' miRNA: 3'- -UGAGGGCGU-CGg--------CAGGGUUGGgCGCU- -5' |
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348 | 3' | -62 | AC_000011.1 | + | 23479 | 0.7 | 0.148747 |
Target: 5'- cCUCCgaCGCGGCCGUCCCAGacaUGCa- -3' miRNA: 3'- uGAGG--GCGUCGGCAGGGUUgg-GCGcu -5' |
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348 | 3' | -62 | AC_000011.1 | + | 3911 | 0.72 | 0.113039 |
Target: 5'- uGCUCCUGCAGCUGagCC-ACCUGCu- -3' miRNA: 3'- -UGAGGGCGUCGGCagGGuUGGGCGcu -5' |
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348 | 3' | -62 | AC_000011.1 | + | 5168 | 1.08 | 0.000168 |
Target: 5'- uACUCCCGCAGCCGUCCCAACCCGCGAa -3' miRNA: 3'- -UGAGGGCGUCGGCAGGGUUGGGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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