Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3480 | 5' | -63.1 | NC_001609.1 | + | 9904 | 0.66 | 0.088105 |
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Target: 5'- aGCACGGCCACCGccuaccggaaGGCACa-UCAu -3' miRNA: 3'- gCGUGCCGGUGGCcg--------CCGUGccAGUu -5' |
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| 3480 | 5' | -63.1 | NC_001609.1 | + | 11148 | 0.66 | 0.082856 |
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Target: 5'- aGCACGGCacagCACUGGCGGCcgcACuGG-CAc -3' miRNA: 3'- gCGUGCCG----GUGGCCGCCG---UG-CCaGUu -5' |
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| 3480 | 5' | -63.1 | NC_001609.1 | + | 6940 | 0.66 | 0.080097 |
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Target: 5'- gCGCuCGGCCACUGGCcgcguauucucccGGCGCuGGggauUCAGg -3' miRNA: 3'- -GCGuGCCGGUGGCCG-------------CCGUG-CC----AGUU- -5' |
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| 3480 | 5' | -63.1 | NC_001609.1 | + | 5590 | 0.67 | 0.073239 |
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Target: 5'- gCGCugGcuugaccguGCCGCCGGUGGCcguGCGGa--- -3' miRNA: 3'- -GCGugC---------CGGUGGCCGCCG---UGCCaguu -5' |
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| 3480 | 5' | -63.1 | NC_001609.1 | + | 6658 | 0.67 | 0.071892 |
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Target: 5'- gGCgGCGGUCgucacugccgggucaGCCGGUGGCAggcugcCGGUCAc -3' miRNA: 3'- gCG-UGCCGG---------------UGGCCGCCGU------GCCAGUu -5' |
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| 3480 | 5' | -63.1 | NC_001609.1 | + | 5257 | 0.67 | 0.071892 |
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Target: 5'- cCGCAUcGCCGCCGGUgauggccuugaguccGGCACcGUCGc -3' miRNA: 3'- -GCGUGcCGGUGGCCG---------------CCGUGcCAGUu -5' |
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| 3480 | 5' | -63.1 | NC_001609.1 | + | 7542 | 0.68 | 0.058932 |
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Target: 5'- aGC-CGGgcagCACCGGUGGCGCGGg--- -3' miRNA: 3'- gCGuGCCg---GUGGCCGCCGUGCCaguu -5' |
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| 3480 | 5' | -63.1 | NC_001609.1 | + | 5545 | 1.06 | 2.8e-05 |
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Target: 5'- cCGCACGGCCACCGGCGGCACGGUCAAg -3' miRNA: 3'- -GCGUGCCGGUGGCCGCCGUGCCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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