Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3481 | 5' | -55.7 | NC_001609.1 | + | 6621 | 0.67 | 0.197721 |
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Target: 5'- cGGCGGCGuucuuccGGGCAGCGuCugUUUCa-- -3' miRNA: 3'- -CCGCCGU-------CCCGUUGUuGugGAAGguu -5' |
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| 3481 | 5' | -55.7 | NC_001609.1 | + | 6408 | 0.67 | 0.192365 |
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Target: 5'- aGGCGGaCAGGuCAGgGGCACCUgUCAc -3' miRNA: 3'- -CCGCC-GUCCcGUUgUUGUGGAaGGUu -5' |
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| 3481 | 5' | -55.7 | NC_001609.1 | + | 5470 | 0.7 | 0.105789 |
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Target: 5'- cGGCGGCAGcGGCAAaaguauCAugGCCgaaauagCCAc -3' miRNA: 3'- -CCGCCGUC-CCGUU------GUugUGGaa-----GGUu -5' |
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| 3481 | 5' | -55.7 | NC_001609.1 | + | 5833 | 0.75 | 0.046423 |
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Target: 5'- cGGCcacaucccgGGCAaaguccuGuGGCGGCAGCACCUUCCAGa -3' miRNA: 3'- -CCG---------CCGU-------C-CCGUUGUUGUGGAAGGUU- -5' |
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| 3481 | 5' | -55.7 | NC_001609.1 | + | 6106 | 1 | 0.000404 |
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Target: 5'- cGGCGGCAGGGCAACAACACCUUCaCAc -3' miRNA: 3'- -CCGCCGUCCCGUUGUUGUGGAAG-GUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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