Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3484 | 3' | -57.9 | NC_001609.1 | + | 5283 | 0.66 | 0.174991 |
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Target: 5'- -aCCGGCGGCGaUGCGGUGUccguugaCCCGa -3' miRNA: 3'- acGGCCGUCGCcAUGCCGUAaa-----GGGU- -5' |
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| 3484 | 3' | -57.9 | NC_001609.1 | + | 8200 | 0.67 | 0.145717 |
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Target: 5'- cUGCCGGUGGUGGa--GGUg--UCCCGu -3' miRNA: 3'- -ACGGCCGUCGCCaugCCGuaaAGGGU- -5' |
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| 3484 | 3' | -57.9 | NC_001609.1 | + | 6577 | 0.67 | 0.136996 |
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Target: 5'- -aCCGGUucCGGUACGGguUUUCgCCAu -3' miRNA: 3'- acGGCCGucGCCAUGCCguAAAG-GGU- -5' |
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| 3484 | 3' | -57.9 | NC_001609.1 | + | 7639 | 0.68 | 0.117254 |
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Target: 5'- -aCCGGCAGCGGUACugaaagaaauGGCcggagaCCCGg -3' miRNA: 3'- acGGCCGUCGCCAUG----------CCGuaaa--GGGU- -5' |
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| 3484 | 3' | -57.9 | NC_001609.1 | + | 10954 | 0.68 | 0.116158 |
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Target: 5'- aGCUGGCAGCGGccgaacaggaauacUGCGGC--UUCUg- -3' miRNA: 3'- aCGGCCGUCGCC--------------AUGCCGuaAAGGgu -5' |
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| 3484 | 3' | -57.9 | NC_001609.1 | + | 6518 | 0.69 | 0.110125 |
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Target: 5'- gUGCCGGuCAGCauc-CGGCA-UUCCCGg -3' miRNA: 3'- -ACGGCC-GUCGccauGCCGUaAAGGGU- -5' |
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| 3484 | 3' | -57.9 | NC_001609.1 | + | 7770 | 0.95 | 0.000711 |
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Target: 5'- aUGuCCgGGCAGCGGUACGGCAUUUCCCAc -3' miRNA: 3'- -AC-GG-CCGUCGCCAUGCCGUAAAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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