Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3487 | 5' | -47.8 | NC_001609.1 | + | 5676 | 0.67 | 0.611749 |
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Target: 5'- gGGUGGAACGUgccguuCUGCGUGucGAGCAc- -3' miRNA: 3'- -CCGUCUUGCG------GACGUACuuUUUGUcc -5' |
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| 3487 | 5' | -47.8 | NC_001609.1 | + | 6207 | 0.68 | 0.547286 |
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Target: 5'- aGGCGGggUGCUucUGCcgGGcAAGGCuGGc -3' miRNA: 3'- -CCGUCuuGCGG--ACGuaCU-UUUUGuCC- -5' |
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| 3487 | 5' | -47.8 | NC_001609.1 | + | 8963 | 0.68 | 0.547286 |
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Target: 5'- cGGCAGcggcaucaguGGCGCaCUGgGgcGGGAGACGGGg -3' miRNA: 3'- -CCGUC----------UUGCG-GACgUa-CUUUUUGUCC- -5' |
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| 3487 | 5' | -47.8 | NC_001609.1 | + | 4427 | 0.7 | 0.437916 |
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Target: 5'- cGGCAGAAUGCCgGagGUGAAGAAUc-- -3' miRNA: 3'- -CCGUCUUGCGGaCg-UACUUUUUGucc -5' |
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| 3487 | 5' | -47.8 | NC_001609.1 | + | 8809 | 0.71 | 0.404293 |
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Target: 5'- uGGCcGGACGCaUUGCcgGAcgcAAACGGGg -3' miRNA: 3'- -CCGuCUUGCG-GACGuaCUu--UUUGUCC- -5' |
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| 3487 | 5' | -47.8 | NC_001609.1 | + | 10725 | 1.15 | 0.000293 |
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Target: 5'- uGGCAGAACGCCUGCAUGAAAAACAGGa -3' miRNA: 3'- -CCGUCUUGCGGACGUACUUUUUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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