Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 349 | 5' | -51.8 | AC_000011.1 | + | 31678 | 0.66 | 0.77931 |
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Target: 5'- -uGGCGCU--GCUGCagGAGCUCGUaGGa -3' miRNA: 3'- cuCCGCGAguUGAUG--CUUGAGUA-CCa -5' |
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| 349 | 5' | -51.8 | AC_000011.1 | + | 7572 | 0.67 | 0.724263 |
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Target: 5'- uGGGCGCUCGagcaGC-ACGGGCUCAg--- -3' miRNA: 3'- cUCCGCGAGU----UGaUGCUUGAGUacca -5' |
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| 349 | 5' | -51.8 | AC_000011.1 | + | 3717 | 0.67 | 0.689874 |
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Target: 5'- uGGGCGCcggCuACUACGGcACUC-UGGUg -3' miRNA: 3'- cUCCGCGa--GuUGAUGCU-UGAGuACCA- -5' |
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| 349 | 5' | -51.8 | AC_000011.1 | + | 20518 | 0.67 | 0.689874 |
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Target: 5'- cGGuGCGCUUGAUUuCGAACUCGuUGGg -3' miRNA: 3'- cUC-CGCGAGUUGAuGCUUGAGU-ACCa -5' |
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| 349 | 5' | -51.8 | AC_000011.1 | + | 4936 | 0.67 | 0.682916 |
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Target: 5'- gGAGGCGCUCuccccccagggaUAgGAGCUCcUGGa -3' miRNA: 3'- -CUCCGCGAGuug---------AUgCUUGAGuACCa -5' |
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| 349 | 5' | -51.8 | AC_000011.1 | + | 35278 | 0.7 | 0.538588 |
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Target: 5'- aGGGCGCUCuGC-GCGAGCucuuuaUCAUGGc -3' miRNA: 3'- cUCCGCGAGuUGaUGCUUG------AGUACCa -5' |
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| 349 | 5' | -51.8 | AC_000011.1 | + | 5391 | 1.07 | 0.00154 |
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Target: 5'- cGAGGCGCUCAACUACGAACUCAUGGUc -3' miRNA: 3'- -CUCCGCGAGUUGAUGCUUGAGUACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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