Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3502 | 3' | -52.3 | NC_001623.1 | + | 113048 | 0.66 | 0.968824 |
Target: 5'- uUCCGucGGCgAGAGAaaaauacgcaucgGGCgaAGCGGCGUg -3' miRNA: 3'- -AGGCuuCCG-UCUUUa------------UCG--UCGUCGCA- -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 100885 | 0.66 | 0.966888 |
Target: 5'- uUUCGGAcGGCGGuucAAUaAGCGGCGcGCGUg -3' miRNA: 3'- -AGGCUU-CCGUCu--UUA-UCGUCGU-CGCA- -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 55713 | 0.66 | 0.959826 |
Target: 5'- gCgGggGGUuGAGGaGGCGGUGGCGg -3' miRNA: 3'- aGgCuuCCGuCUUUaUCGUCGUCGCa -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 94818 | 0.67 | 0.951789 |
Target: 5'- -aCGGAGGUuu---UAGCAGCGGCa- -3' miRNA: 3'- agGCUUCCGucuuuAUCGUCGUCGca -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 69987 | 0.67 | 0.951789 |
Target: 5'- cUCgGgcGGUGGAGGUGGUgcgguuGGCGGCGc -3' miRNA: 3'- -AGgCuuCCGUCUUUAUCG------UCGUCGCa -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 34810 | 0.67 | 0.94274 |
Target: 5'- -gUGAAGGCu---GUGGCAGC-GCGUg -3' miRNA: 3'- agGCUUCCGucuuUAUCGUCGuCGCA- -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 36974 | 0.67 | 0.932653 |
Target: 5'- -----cGGCAGugGUAGUAGUAGCGg -3' miRNA: 3'- aggcuuCCGUCuuUAUCGUCGUCGCa -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 78165 | 0.68 | 0.927216 |
Target: 5'- cCCaAAGGacugGGAGAaGGCGGCGGCGa -3' miRNA: 3'- aGGcUUCCg---UCUUUaUCGUCGUCGCa -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 87587 | 0.68 | 0.915551 |
Target: 5'- uUCgGAAGGCugcGcgGgcGCGGCGGCGUu -3' miRNA: 3'- -AGgCUUCCGu--CuuUauCGUCGUCGCA- -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 55798 | 0.69 | 0.874364 |
Target: 5'- uUCUGgcGGCuGuguUGGCGGCGGUGUc -3' miRNA: 3'- -AGGCuuCCGuCuuuAUCGUCGUCGCA- -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 78206 | 0.7 | 0.833467 |
Target: 5'- --aGGAGGaauaGGAGAaGGCGGCGGCGa -3' miRNA: 3'- aggCUUCCg---UCUUUaUCGUCGUCGCa -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 16392 | 0.71 | 0.786587 |
Target: 5'- aCCGAAgugguGGUAGAAGUgguagaaGGCGGguGCGUu -3' miRNA: 3'- aGGCUU-----CCGUCUUUA-------UCGUCguCGCA- -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 78243 | 0.72 | 0.737789 |
Target: 5'- gUUGGAGGaauaGGAGAaGGCGGCGGCGa -3' miRNA: 3'- aGGCUUCCg---UCUUUaUCGUCGUCGCa -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 6060 | 0.83 | 0.218144 |
Target: 5'- -gCGGAGGCGGAGGUGGUGGCGGUGa -3' miRNA: 3'- agGCUUCCGUCUUUAUCGUCGUCGCa -5' |
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3502 | 3' | -52.3 | NC_001623.1 | + | 16599 | 1.09 | 0.004905 |
Target: 5'- cUCCGAAGGCAGAAAUAGCAGCAGCGUg -3' miRNA: 3'- -AGGCUUCCGUCUUUAUCGUCGUCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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